PMID- 9287970
OWN - NLM
STAT- MEDLINE
DCOM- 19970930
LR  - 20190707
IS  - 0016-5085 (Print)
IS  - 0016-5085 (Linking)
VI  - 113
IP  - 3
DP  - 1997 Sep
TI  - Chromosomal alterations in ulcerative colitis-related neoplastic progression.
PG  - 791-801
AB  - BACKGROUND & AIMS: It is unclear whether genomic derangement precedes the 
      histological development of dysplasia in ulcerative colitis (UC)-related 
      neoplastic progression. The primary aim of this study was to determine if 
      chromosomal alterations occur early in the progression pathway of UC-related 
      neoplasia. METHODS: Fluorescence in situ hybridization (FISH) was performed on 
      nuclei dissociated from sites of cancer, dysplasia, and UC-involved nondysplastic 
      epithelium in five UC-related cancer colectomy specimens using a panel of 
      pericentromeric probes. Comparative genomic hybridization (CGH) was used to 
      detect clonal chromosomal losses and gains in DNA extracted from these sites. 
      RESULTS: FISH analysis revealed significant and often dramatic alterations in 
      chromosome copy number compared with controls in all biopsy specimens of cancer, 
      dysplasia, and nondysplastic UC-involved epithelium. Clonal chromosomal losses 
      and gains were detected by CGH in all but one analyzed site of dysplasia and 
      cancer and in two of the five nondysplastic sites. FISH and CGH frequently 
      detected the relative loss of chromosome 18. CONCLUSIONS: Chromosomal alterations 
      may occur early in UC-related neoplastic progression and seem to precede the 
      histological development of dysplasia. Relative loss of 18q may be important in 
      the progression of UC-related neoplasia. The detection of chromosomal alterations 
      as an intermediate end point may prove useful in identifying patients at high 
      risk for the development of colorectal cancer.
FAU - Willenbucher, R F
AU  - Willenbucher RF
AD  - Cancer Center, University of California, San Francisco, USA.
FAU - Zelman, S J
AU  - Zelman SJ
FAU - Ferrell, L D
AU  - Ferrell LD
FAU - Moore, D H 2nd
AU  - Moore DH 2nd
FAU - Waldman, F M
AU  - Waldman FM
LA  - eng
GR  - CA44768/CA/NCI NIH HHS/United States
GR  - CA47537/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Gastroenterology
JT  - Gastroenterology
JID - 0374630
SB  - IM
CIN - Gastroenterology. 1998 Mar;114(3):624. PMID: 9496964
MH  - Adult
MH  - Chromosome Aberrations/*genetics
MH  - Chromosome Deletion
MH  - Chromosome Disorders
MH  - Colitis, Ulcerative/*complications/pathology
MH  - Colon/pathology
MH  - Colorectal Neoplasms/*complications/*pathology
MH  - Disease Progression
MH  - Epithelium/pathology
MH  - Female
MH  - Gene Dosage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Nucleic Acid Hybridization
EDAT- 1997/09/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1997/09/01 00:00
PHST- 1997/09/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1997/09/01 00:00 [entrez]
AID - S0016508597004162 [pii]
AID - 10.1016/s0016-5085(97)70173-2 [doi]
PST - ppublish
SO  - Gastroenterology. 1997 Sep;113(3):791-801. doi: 10.1016/s0016-5085(97)70173-2.