PMID- 9288127
OWN - NLM
STAT- MEDLINE
DCOM- 19971002
LR  - 20210102
IS  - 0039-6060 (Print)
IS  - 0039-6060 (Linking)
VI  - 122
IP  - 2
DP  - 1997 Aug
TI  - Active immunization using dendritic cells mixed with tumor cells inhibits the 
      growth of primary breast cancer.
PG  - 228-34
AB  - BACKGROUND: Dendritic cells (DCs) are potent antigen-presenting cells regarded as 
      crucial in the priming of an immune response. The goal of our study was to test 
      whether bone marrow-generated DCs are capable of inducing protective immunity 
      against a murine breast carcinoma (4T1). METHODS: DCs were grown from Balb/c mice 
      by culturing lymphocyte-immunodepleted bone marrow in murine 
      granulocyte-macrophage colony-stimulating factor containing medium for 10 days. 
      Balb/c mice (five to eight per group) were immunized intradermally with 1 x 10(6) 
      DCs mixed with 2 x 10(6) lethally irradiated 4T1 cells on day 0. Mice in control 
      groups were given intradermal inoculations of phosphate-buffered saline solution, 
      1 x 10(6) DCs, or lethally irradiated 4T1 cells alone. Booster intraperitoneal 
      immunizations of 2 x 10(6) lethally irradiated 4T1 cells were given on days 7 and 
      14. All mice were challenged with 5 x 10(3) 4T1 cells subcutaneously 7 days after 
      the final immunization. Animals were examined daily, and tumor volume was 
      recorded twice weekly with calipers. RESULTS: At 21 days there was a significant 
      reduction in tumor growth in mice immunized with DCs mixed with irradiated 4T1 
      cells as compared with the control groups (p = 0.0005, Kruskal-Wallis test). 
      CONCLUSIONS: These results suggest that DCs mixed with tumor cells as a source of 
      undefined tumor antigen can induce an effective antitumor immune response. This 
      finding provides a rationale for the use of cultured DCs in immunotherapy of 
      breast and other cancers.
FAU - Coveney, E
AU  - Coveney E
AD  - Department of Surgery, Duke University Medical Center, Durham, N.C. 27710, USA.
FAU - Wheatley, G H 3rd
AU  - Wheatley GH 3rd
FAU - Lyerly, H K
AU  - Lyerly HK
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Surgery
JT  - Surgery
JID - 0417347
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Bone Marrow/drug effects
MH  - *Bone Marrow Cells
MH  - Cell Culture Techniques/methods
MH  - Cell Differentiation
MH  - Coculture Techniques
MH  - Cytotoxicity, Immunologic
MH  - Dendritic Cells/cytology/immunology/*transplantation
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/*pharmacology
MH  - *Immunotherapy, Active
MH  - Lymphocyte Depletion
MH  - Male
MH  - Mammary Neoplasms, Experimental/*immunology/*pathology/therapy
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Phenotype
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Tumor Cells, Cultured
EDAT- 1997/08/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
AID - S0039-6060(97)90013-1 [pii]
AID - 10.1016/s0039-6060(97)90013-1 [doi]
PST - ppublish
SO  - Surgery. 1997 Aug;122(2):228-34. doi: 10.1016/s0039-6060(97)90013-1.