PMID- 9288554
OWN - NLM
STAT- MEDLINE
DCOM- 19971023
LR  - 20191210
IS  - 0910-8327 (Print)
IS  - 0910-8327 (Linking)
VI  - 12
IP  - 1
DP  - 1997
TI  - Differences in sarcoplasmic reticulum gene expression in myocardium from patients 
      undergoing cardiac surgery. Quantification of steady-state levels of messenger 
      RNA using the reverse transcription-polymerase chain reaction.
PG  - 1-9
AB  - Little is known about any alterations in sarcoplasmic reticulum (SR) gene 
      expression associated with cardiac diseases of varying degrees of severity. We 
      assessed, using the reverse transcription-polymerase chain reaction (RT-PCR) 
      technique, SR Ca2+ transport protein gene expression in small tissue samples from 
      failing hearts in patients undergoing cardiac surgery. Total RNA was extracted 
      from 30- to 50-mg samples from the hearts of 13 patients with coronary artery 
      disease, congenital heart disease, or valvular heart disease. We used RT-PCR to 
      synthesize and amplify cDNA encoding cardiac SR Ca(2+)-ATPase, ryanodine receptor 
      (RYR), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The amount of each 
      mRNA in the sample was expressed relative to the amount of GAPDH mRNA. The 
      expression level of each mRNA was correlated with the cardiac functional index. 
      The mRNA levels for Ca(2+)-ATPase and RYR varied between heart samples, but 
      showed a positive correlation with left ventricular ejection fraction. 
      Ca(2+)-ATPase mRNA levels showed in inverse relationship with plasma brain 
      natriuretic peptide. In addition, we isolated partial cDNA encoding a human 
      cardiac RYR. The cDNA consisted of 487 nucleotides, and the nucleotide and 
      deduced amino acid sequences showed 93% and 99% homology, respectively, to those 
      of rabbit cardiac RYR. These results suggest that decreased levels of mRNA for SR 
      Ca2+ transport protein could be related to abnormal cardiac function, regardless 
      of the etiology of the heart disease. RT-PCR provides a rapid and economical way 
      of quantifying the expression of multiple genes in small specimens and may, 
      therefore, aid understanding of the pathophysiology and treatment of heart 
      disease.
FAU - Ohkusa, T
AU  - Ohkusa T
AD  - Second Department of Internal Medicine, Yamaguchi University School of Medicine, 
      Japan.
FAU - Noma, T
AU  - Noma T
FAU - Ueyama, T
AU  - Ueyama T
FAU - Hisamatsu, Y
AU  - Hisamatsu Y
FAU - Yano, M
AU  - Yano M
FAU - Esato, K
AU  - Esato K
FAU - Nakazawa, A
AU  - Nakazawa A
FAU - Matsuzaki, M
AU  - Matsuzaki M
LA  - eng
SI  - GENBANK/D83212
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Heart Vessels
JT  - Heart and vessels
JID - 8511258
RN  - 0 (Calcium Channels)
RN  - 0 (Muscle Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Ryanodine Receptor Calcium Release Channel)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Calcium Channels/*genetics
MH  - Calcium-Transporting ATPases/*genetics
MH  - Cardiac Surgical Procedures
MH  - Child
MH  - Female
MH  - *Gene Expression
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism
MH  - Heart Diseases/*metabolism/pathology/surgery
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Muscle Proteins/*genetics
MH  - Myocardium/*chemistry/metabolism
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/*analysis
MH  - Ryanodine Receptor Calcium Release Channel
MH  - Sarcoplasmic Reticulum/*genetics/metabolism
MH  - Transcription, Genetic
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1007/BF01747496 [doi]
PST - ppublish
SO  - Heart Vessels. 1997;12(1):1-9. doi: 10.1007/BF01747496.