PMID- 9290144
OWN - NLM
STAT- MEDLINE
DCOM- 19971015
LR  - 20190920
IS  - 0143-4004 (Print)
IS  - 0143-4004 (Linking)
VI  - 18
IP  - 7
DP  - 1997 Sep
TI  - Cellular localization and developmental regulation of 11 beta-hydroxysteroid 
      dehydrogenase type 1 (11 beta-HSD1) gene expression in the ovine placenta.
PG  - 503-9
AB  - This study was designed to examine the cellular localization and developmental 
      regulation of 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD) type 1 gene 
      expression in the ovine placenta. Placental tissues were collected at discrete 
      times between days 59 and 143 of pregnancy (term = 145 days). Levels of 11 
      beta-HSD1 mRNA were determined by Norther blot analysis. The level of both 
      dehydrogenase and reductase activities of 11 beta-HSD1 was assessed by a 
      radiometric conversion assay using cortisol and cortisone as physiological 
      substrates. The cellular localization of 11 beta-HSD1 protein was determined by 
      standard immunohistochemical technique using a polyclonal antibody specific for 
      the ovine protein. High levels of 11 beta-HSD1 mRNA were detected in the placenta 
      by day 59, and there was a trend towards a decrease between days 98-103 and 
      125-128 (P = 0.06). The level of placental 11 beta-HSD1 mRNA remained unchanged 
      thereafter. Levels of both 11 beta-HSD1 dehydrogenase and reductase activities 
      followed a similar pattern except that in both cases there was a significant 
      decrease between 98-103 and 125-128 (P < 0.05). Moreover, under the present assay 
      conditions, the dehydrogenase activity was always predominant, suggesting that 
      the net effect of placental 11 beta-HSD1 activity would lead to glucocorticoid 
      inactivation. Thus, the decreased 11 beta-HSD1 activity in the placenta at days 
      125-128 was consistent with, and may help to explain, the apparent increase in 
      the placental transfer of cortisol from mother to fetus during that time. 
      Throughout pregnancy, intense 11 beta-HSD1 immunoreactivity was detected in fetal 
      trophoblastic cells, maternal stromal cells and blood vessels. In contrast, 
      maternal syncytium was immunonegative before day 125, but became immunopositive 
      thereafter. The observed predominant direction of 11 beta-HSD1 activity in vitro 
      and its pattern of localization in the ovine placenta are consistent with the 
      hypothesis that placental 11 beta-HSD protects the fetus from adverse effects of 
      maternal glucocorticoids by inactivating glucocorticoids locally.
FAU - Yang, K
AU  - Yang K
AD  - Lawson Research Institute, St Joseph's Hospital, Department of Ob/Gyn, University 
      of Western Ontario, London, Canada.
FAU - Langlois, D A
AU  - Langlois DA
FAU - Campbell, L E
AU  - Campbell LE
FAU - Challis, J R
AU  - Challis JR
FAU - Krkosek, M
AU  - Krkosek M
FAU - Yu, M
AU  - Yu M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Placenta
JT  - Placenta
JID - 8006349
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - Animals
MH  - Blotting, Northern
MH  - Female
MH  - *Gene Expression Regulation, Developmental
MH  - Hydroxysteroid Dehydrogenases/analysis/*genetics
MH  - Isoenzymes/*genetics
MH  - Placenta/*enzymology
MH  - Pregnancy
MH  - RNA, Messenger/*analysis/metabolism
MH  - Sheep
EDAT- 1997/09/18 00:00
MHDA- 1997/09/18 00:01
CRDT- 1997/09/18 00:00
PHST- 1997/09/18 00:00 [pubmed]
PHST- 1997/09/18 00:01 [medline]
PHST- 1997/09/18 00:00 [entrez]
AID - 0143-4004(77)90003-0 [pii]
AID - 10.1016/0143-4004(77)90003-0 [doi]
PST - ppublish
SO  - Placenta. 1997 Sep;18(7):503-9. doi: 10.1016/0143-4004(77)90003-0.