PMID- 9290951
OWN - NLM
STAT- MEDLINE
DCOM- 19971016
LR  - 20061115
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 20
IP  - 1
DP  - 1997 Sep
TI  - Molecular cytogenetic analysis of a nontumorigenic human breast epithelial cell 
      line that eventually turns tumorigenic: validation of an analytical approach 
      combining karyotyping, comparative genomic hybridization, chromosome painting, 
      and single-locus fluorescence in situ hybridization.
PG  - 30-7
AB  - The immortalized, nontumorigenic human breast epithelial cell line HMT-3522 has 
      been used as a model for premalignant and, eventually, malignant development. 
      During cultivation, the karyotype evolution was followed. At an early stage, a 
      very long constant phase showed a near-diploid karyotype, with only five marker 
      chromosomes. DNA from this phase was used for comparative genomic hybridization 
      (CGH) analysis, confirming a previously known MYC amplification, and the 
      integration sites were subsequently determined by single-locus fluorescence in 
      situ hybridization (FISH). Furthermore, gains of 5q22-qter and 20q11-qter and 
      deletion of most of chromosome 6 (6p23-qter) were detected by CGH. Because of 
      uncertainty about some of the indicated changes, including a deletion of 
      Ip35-pter, the CGH findings were investigated more closely by chromosome 
      painting, leading to a revision of the karyotype: 
      45,XX,del(I)(p35),-6,dup(8)(pter-->qter::qter-->q24),der(12) t(6;12)(p23; 
      p13),der(14)t(5;14)(q22;q32.3),der(17)t(8;17;20)(17pter-->17q25 ::8qter--> 
      8q23::8q24-->8qter::8q24-->8qter:: 8q23-->8q24.1::20q11-->20qter). Some 
      karyotypic changes were confirmed by CGH; others had to be revised; and, in the 
      Ip35 region, classical cytogenetics seems superior to CGH. However, CGH revealed 
      a karyotypically unsuspected dup(20q) that might be of special relevance to 
      breast tumor initiation or progression. Our study confirms that CGH is 
      supplementary to current technologies, e.g., karyotyping and Southern analysis, 
      but cannot replace them. In addition, our cell line turned out to be an excellent 
      model for comparison among the different methods. The results imply that future 
      cytogenetic analyses of complex karyotypes should be based on a combination of 
      karyotyping, CGH, and FISH.
FAU - Nielsen, K V
AU  - Nielsen KV
AD  - Department of Medical Genetics, Panum Institute, University of Copenhagen, 
      Denmark. kirsten.vang@dako.dk
FAU - Niebuhr, E
AU  - Niebuhr E
FAU - Ejlertsen, B
AU  - Ejlertsen B
FAU - Holstebroe, S
AU  - Holstebroe S
FAU - Madsen, M W
AU  - Madsen MW
FAU - Briand, P
AU  - Briand P
FAU - Mouridsen, H T
AU  - Mouridsen HT
FAU - Bolund, L
AU  - Bolund L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - Breast/*cytology
MH  - Breast Neoplasms/*genetics/pathology
MH  - Cell Line
MH  - Cell Transformation, Neoplastic/*genetics
MH  - Chromosome Mapping/methods
MH  - Chromosomes, Human, Pair 8/genetics
MH  - *Cytogenetics
MH  - Gene Amplification
MH  - *Genes, myc
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Nucleic Acid Hybridization
EDAT- 1997/09/18 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/09/18 00:00
PHST- 1997/09/18 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/09/18 00:00 [entrez]
AID - 10.1002/(SICI)1098-2264(199709)20:1<30::AID-GCC5>3.0.CO;2-A [pii]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1997 Sep;20(1):30-7.