PMID- 9290987
OWN - NLM
STAT- MEDLINE
DCOM- 19971022
LR  - 20190826
IS  - 0342-4642 (Print)
IS  - 0342-4642 (Linking)
VI  - 23
IP  - 7
DP  - 1997 Jul
TI  - A controlled study of leukocyte activation in septic patients.
PG  - 743-8
AB  - OBJECTIVE: Qualitative and quantitative evaluation of leukocyte activation in 
      septic patients in comparison to two control groups. DESIGN: A prospective 
      clinical study in which the leukocyte oxidative output of whole blood was 
      measured in three groups of patients. Two chemiluminescence markers (luminol or 
      lucigenin), indicative of either total oxidant output or superoxide production, 
      and three stimuli (opsonized zymosan, formyl-methionyl-leucyl-phenylalanine 
      (fMLP), phorbol myristate acetate) (PMA), representing different pathways of 
      leukocyte activation, were used. Tumor necrosis factor, interleukin-6 and 
      C-reactive protein (TNF, IL-6, and CRP) were determined to evaluate the severity 
      of the inflammatory process. SETTING: Intensive care and surgical units of a 
      university hospital. PATIENTS: Seventy-four healthy patients, ten ICU patients 
      without signs of sepsis or systemic inflammatory response syndrome and 19 septic 
      patients were studied. MEASUREMENT AND MAIN RESULTS: With all three stimuli, 
      whole blood total oxidative output and superoxide production were generally 
      increased in septic patients. This was most likely due to the increased leukocyte 
      numbers in these patients. When the chemiluminescence values were normalized per 
      phagocyte (granulocytes and monocytes), the total oxidative output of septic 
      phagocytes decreased with opsonin and fMLP but increased with PMA, while 
      superoxide output decreased regardless of the stimuli used. TNF, IL-6 and CRP, 
      although increased in septic patients as compared to ICU controls, correlated 
      weakly with oxidant output. CONCLUSIONS: The oxidative output of whole blood was 
      increased in septic patients compared to controls because of elevated leukocyte 
      numbers. However, oxidant output normalized for phagocyte numbers generally 
      decreases during sepsis for most stimuli. Cytokines and CRP do not appear to be 
      associated with the extent of oxidant output during sepsis.
FAU - Pascual, C
AU  - Pascual C
AD  - Department of Anesthesiology and Intensive Care Medicine, Friedrich Schiller 
      University, Jena, Germany.
FAU - Karzai, W
AU  - Karzai W
FAU - Meier-Hellmann, A
AU  - Meier-Hellmann A
FAU - Bredle, D L
AU  - Bredle DL
FAU - Reinhart, K
AU  - Reinhart K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Intensive Care Med
JT  - Intensive care medicine
JID - 7704851
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9007-41-4 (C-Reactive Protein)
SB  - IM
MH  - Adult
MH  - Aged
MH  - C-Reactive Protein/*metabolism
MH  - Case-Control Studies
MH  - Female
MH  - Humans
MH  - Interleukin-6/*blood
MH  - Leukocyte Count
MH  - Male
MH  - Middle Aged
MH  - *Neutrophil Activation
MH  - Prospective Studies
MH  - *Respiratory Burst
MH  - Sepsis/*immunology/*metabolism
MH  - Severity of Illness Index
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 1997/07/01 00:00
MHDA- 1997/09/18 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1997/09/18 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
AID - 10.1007/s001340050403 [doi]
PST - ppublish
SO  - Intensive Care Med. 1997 Jul;23(7):743-8. doi: 10.1007/s001340050403.