PMID- 9291152
OWN - NLM
STAT- MEDLINE
DCOM- 19971023
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 231
IP  - 2
DP  - 1997 Aug 8
TI  - Gene expression of catecholamine synthesizing enzymes and beta adrenoceptor 
      subtypes during rat embryogenesis.
PG  - 108-12
AB  - Timed-pregnant Sprague-Dawley rats were killed between gestational day (GD) 8 and 
      10, and embryos were explanted and separated into developmental stages according 
      to a modified Theiler's system. Total RNA from each stage was isolated and 
      subjected to reverse transcription-polymerase chain reaction (RT-PCR) assays to 
      examine gene expression of catecholamine synthesizing enzymes and three subtypes 
      of beta adrenoceptors. Expression of these genes was detected at much earlier 
      stages than previously reported, and each enzyme and receptor subtype showed a 
      different pattern of gene expression. For example, mRNA for tyrosine hydroxylase, 
      the rate-limiting enzyme for catecholamine synthesis, was detected as early as 
      stage 10a, late GD 8, before the neural crest cells appear (stage 12, mid GD 10). 
      This contradicts the common belief that catecholamines are produced only in the 
      cells of sympathoadrenal lineage which originate from the neural crest cells and 
      the cells of central nervous system. Results from the present study indicate that 
      catecholamine synthesis is not limited to the cells of sympathoadrenal lineage.
FAU - Fujinaga, M
AU  - Fujinaga M
AD  - Department of Anesthesia, Stanford University School of Medicine, CA 94305, USA. 
      fujinaga@leland.stanford.edu
FAU - Scott, J C
AU  - Scott JC
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Catecholamines)
RN  - 0 (DNA Primers)
RN  - 0 (Enzymes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Adrenergic, beta)
SB  - IM
MH  - Animals
MH  - Catecholamines/biosynthesis/*metabolism
MH  - DNA Primers
MH  - Embryo, Mammalian/chemistry/enzymology/physiology
MH  - Enzymes/genetics/metabolism
MH  - Female
MH  - Gene Expression Regulation, Developmental/physiology
MH  - Gene Expression Regulation, Enzymologic/physiology
MH  - Nervous System/chemistry/*embryology/*enzymology
MH  - Pregnancy
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Adrenergic, beta/*genetics
EDAT- 1997/08/08 00:00
MHDA- 1997/09/18 00:01
CRDT- 1997/08/08 00:00
PHST- 1997/08/08 00:00 [pubmed]
PHST- 1997/09/18 00:01 [medline]
PHST- 1997/08/08 00:00 [entrez]
AID - S0304-3940(97)00511-9 [pii]
AID - 10.1016/s0304-3940(97)00511-9 [doi]
PST - ppublish
SO  - Neurosci Lett. 1997 Aug 8;231(2):108-12. doi: 10.1016/s0304-3940(97)00511-9.