PMID- 9291471
OWN - NLM
STAT- MEDLINE
DCOM- 19971202
LR  - 20131121
IS  - 1040-452X (Print)
IS  - 1040-452X (Linking)
VI  - 48
IP  - 2
DP  - 1997 Oct
TI  - Glucose metabolism during bovine preimplantation development: analysis of gene 
      expression in single oocytes and embryos.
PG  - 216-26
AB  - Glucose metabolism of the bovine embryo is low during the first cleavages and 
      increases sharply after the major resumption of the genome (8-16 cells). The mRNA 
      level for genes involved in glucose metabolism was tested by RT-PCR on individual 
      oocytes and embryos at different stages of development. These genes were: glucose 
      transport GLUT-1, hexokinase (HK), glucose-6-phosphatase-dehydrogenase (G6PDH), 
      and glucose-phosphate-isomerase (GPI); actin was used as a reference transcript. 
      RT-PCR results revealed three types of oocytes or embryos: positive with a PCR 
      signal for each transcript considered, nul with no signal for any transcript, and 
      heterogeneous with a PCR signal for some transcripts and none for others. The 
      number of nul and heterogeneous samples was higher for slow than for 
      fast-cleaving embryos (81% vs. 36%), and the proportion of positive embryos 
      increased significantly at the 16-cell and morula stages (P < 0.002), suggesting 
      a correlation between mRNA content and developmental capacity. In positive 
      embryos, GLUT-1 level was reduced by half during maturation and fertilization. 
      Actin and hexokinase mRNA levels decreased during the first cleavages, but 
      significantly increased at the 16-cell and morula stages, respectively. GPI 
      transcript remained stable throughout development, whereas there was a 
      significant rise for G6PDH at the 4-cell stage, perhaps due to a polyadenylation 
      process. Finally, the absence or decrease in intensity of several transcripts at 
      the blastocyst stage suggests suboptimal culture conditions.
FAU - Lequarre, A S
AU  - Lequarre AS
AD  - Unite des Sciences Veterinaires, Universite Catholique de Louvain, 
      Louvain-la-Neuve, Belgium.
FAU - Grisart, B
AU  - Grisart B
FAU - Moreau, B
AU  - Moreau B
FAU - Schuurbiers, N
AU  - Schuurbiers N
FAU - Massip, A
AU  - Massip A
FAU - Dessy, F
AU  - Dessy F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (Actins)
RN  - 0 (Glucose Transporter Type 1)
RN  - 0 (Monosaccharide Transport Proteins)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 5.3.1.9 (Glucose-6-Phosphate Isomerase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Actins/genetics
MH  - Animals
MH  - Cattle
MH  - Embryo, Mammalian/*metabolism
MH  - *Embryonic Development
MH  - Female
MH  - *Gene Expression Regulation, Developmental
MH  - *Gene Expression Regulation, Enzymologic
MH  - Glucose/*metabolism
MH  - Glucose Transporter Type 1
MH  - Glucose-6-Phosphate Isomerase/genetics
MH  - Glucosephosphate Dehydrogenase/genetics
MH  - Hexokinase/genetics
MH  - Monosaccharide Transport Proteins/genetics
MH  - Oocytes/*metabolism
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - Transcription, Genetic
EDAT- 1997/09/18 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/09/18 00:00
PHST- 1997/09/18 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/09/18 00:00 [entrez]
AID - 10.1002/(SICI)1098-2795(199710)48:2<216::AID-MRD9>3.0.CO;2-V [pii]
AID - 10.1002/(SICI)1098-2795(199710)48:2<216::AID-MRD9>3.0.CO;2-V [doi]
PST - ppublish
SO  - Mol Reprod Dev. 1997 Oct;48(2):216-26. doi: 
      10.1002/(SICI)1098-2795(199710)48:2<216::AID-MRD9>3.0.CO;2-V.