PMID- 9292014
OWN - NLM
STAT- MEDLINE
DCOM- 19971001
LR  - 20191210
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 78 ( Pt 9)
DP  - 1997 Sep
TI  - Epitope mapping of the nucleocapsid protein of European and North American 
      isolates of porcine reproductive and respiratory syndrome virus.
PG  - 2269-78
AB  - Two major genotypes of porcine reproductive and respiratory syndrome virus 
      (PRRSV) have been described, which correspond to the European and North American 
      isolates. PRRSV nucleocapsid (N) protein has been identified as the most 
      immunodominant viral protein. The N genes from two PRRSV isolates, Olot/91 
      (European) and Quebec 807/94 (North American), were cloned and expressed in: (i) 
      baculovirus under the control of the polyhedrin promoter and (ii) Escherichia 
      coli using the pET3x system. The N protein from both isolates was expressed much 
      more efficiently in E. coli as a fusion protein than in baculovirus. The 
      antigenicity of the protein was similar in both systems and it was recognized by 
      a collection of 48 PRRSV-positive pig sera. The antigenic structure of the PRRSV 
      N protein was investigated using seven monoclonal antibodies (MAbs) and 
      overlapping fragments of the protein expressed in E. coli. Four MAbs recognized 
      two discontinuous epitopes that were present in the partially folded protein, or 
      at least a large fragment comprising the first 78 residues. The other three MAbs 
      revealed the presence of a common antigenic site localized in the central region 
      of the protein (amino acids 50-66). This region is well conserved among different 
      isolates of European and North American origin and is the most hydrophilic region 
      of the protein. However, this epitope, although recognized by the MAbs and many 
      pig sera, is not useful for diagnostic purposes. Moreover, none of the N protein 
      fragments were able to mimic the antigenicity of the entire protein.
FAU - Rodriguez, M J
AU  - Rodriguez MJ
AD  - INGENASA, Madrid, Spain.
FAU - Sarraseca, J
AU  - Sarraseca J
FAU - Garcia, J
AU  - Garcia J
FAU - Sanz, A
AU  - Sanz A
FAU - Plana-Duran, J
AU  - Plana-Duran J
FAU - Ignacio Casal, J
AU  - Ignacio Casal J
LA  - eng
SI  - GENBANK/U00153
SI  - GENBANK/Z82995
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Immune Sera)
RN  - 0 (Immunodominant Epitopes)
RN  - 0 (Recombinant Fusion Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Cloning, Molecular
MH  - *Epitope Mapping
MH  - Escherichia coli/genetics
MH  - Europe
MH  - Genetic Vectors
MH  - Immune Sera
MH  - Immunodominant Epitopes/analysis/chemistry
MH  - Molecular Sequence Data
MH  - Nucleocapsid/*chemistry/genetics/immunology
MH  - Nucleopolyhedroviruses/genetics
MH  - Open Reading Frames/genetics
MH  - Porcine respiratory and reproductive syndrome 
      virus/*chemistry/genetics/immunology
MH  - Protein Conformation
MH  - Quebec
MH  - Recombinant Fusion Proteins
MH  - Sequence Analysis, DNA
MH  - Swine
EDAT- 1997/09/18 00:00
MHDA- 1997/09/18 00:01
CRDT- 1997/09/18 00:00
PHST- 1997/09/18 00:00 [pubmed]
PHST- 1997/09/18 00:01 [medline]
PHST- 1997/09/18 00:00 [entrez]
AID - 10.1099/0022-1317-78-9-2269 [doi]
PST - ppublish
SO  - J Gen Virol. 1997 Sep;78 ( Pt 9):2269-78. doi: 10.1099/0022-1317-78-9-2269.