PMID- 9292235
OWN - NLM
STAT- MEDLINE
DCOM- 19971023
LR  - 20181113
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 5
IP  - 5
DP  - 1997 Aug
TI  - Primed in situ (PRINS) labelling with Alu and satellite primers for rapid 
      characterization of human chromosomes in hybrid cell lines.
PG  - 307-12
AB  - The primed in situ (PRINS) labelling method was developed as an alternative to 
      classical cytogenetics and fluorescence in situ hybridization (FISH) for the 
      characterization of interspecific somatic hybrids. Full karyotypes were performed 
      by PRINS using Alu-specific primers to generate the painting of all human 
      material associated with R-like banding. The representativity of individual human 
      chromosomes was established using primers specific for discriminent 
      alpha-satellite DNA sequences providing specific signals on the centromeres of 
      the targeted chromosomes and corresponding spots in interphase nuclei. Using this 
      methodology, a somatic hybrid clone was shown to be monochromosomal for the 
      der(11) from a t(11;22) patient.
FAU - Coullin, P
AU  - Coullin P
AD  - INSERM U178, Villejuif, France.
FAU - Andreo, B
AU  - Andreo B
FAU - Charlieu, J P
AU  - Charlieu JP
FAU - Candelier, J J
AU  - Candelier JJ
FAU - Pellestor, F
AU  - Pellestor F
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (DNA Primers)
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - Cells, Cultured
MH  - Centromere/genetics
MH  - Chromosome Banding
MH  - Chromosomes, Human/*genetics
MH  - Cricetinae
MH  - DNA Primers/genetics
MH  - Fibroblasts
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - Interphase/genetics
MH  - Karyotyping
MH  - Mice
MH  - *Microsatellite Repeats
EDAT- 1997/08/01 00:00
MHDA- 1997/09/18 00:01
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 1997/09/18 00:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
AID - 5384135 [pii]
AID - 10.1023/B:CHRO.0000038761.15856.4d [doi]
PST - ppublish
SO  - Chromosome Res. 1997 Aug;5(5):307-12. doi: 10.1023/B:CHRO.0000038761.15856.4d.