PMID- 9292513
OWN - NLM
STAT- MEDLINE
DCOM- 19970930
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 90
IP  - 5
DP  - 1997 Sep 1
TI  - Expression of functional TrkA receptor tyrosine kinase in the HMC-1 human mast 
      cell line and in human mast cells.
PG  - 1807-20
AB  - Nerve growth factor (NGF) can influence mast cell development and function in 
      murine rodents by interacting with its receptors on mast cells. We now report the 
      identification of mRNA transcripts of full-length tyrosine kinase-containing 
      trkA, trkB, and trkC neurotrophin receptor genes in HMC-1 human mast cell 
      leukemia cells. Although HMC-1 cells lacked p75 mRNA, they expressed transcripts 
      for the exon-lacking splice variant of trkA (trkAI), truncated trkB (trkB.T1), 
      and truncated trkC. By flow cytometry, HMC-1 cells exhibited expression of TrkA, 
      TrkB, and TrkC receptor proteins containing full-length tyrosine kinase domains. 
      NGF stimulation of HMC-1 cells induced tyrosine phosphorylation of TrkA protein, 
      increased expression of the early response genes c-fos and NGF1-A, and activation 
      of ERK-mitogen-activated protein (MAP) kinase, results which indicate that TrkA 
      receptors in HMC-1 cells are fully functional. Highly purified populations of 
      human lung mast cells expressed mRNAs for trkA, trkB and trkC, whereas 
      preparations of human umbilical cord blood-derived mast cells expressed mRNAs for 
      trkA and trkC, but not trkB. Moreover, preparations of human umbilical cord 
      blood-derived immature mast cells not only expressed mRNA transcript and protein 
      for TrkA, but exhibited significantly higher numbers of chymase-positive cells 
      after the addition of NGF to their culture medium for 3 weeks. In addition, HMC-1 
      cells expressed mRNAs for NGF, brain-derived neurotrophic factor (BDNF), and 
      neurotrophin-3 (NT-3), the cognate ligands for TrkA, TrkB, and TrkC, whereas NGF 
      and BDNF transcripts were detectable in human umbilical cord blood mast cell 
      preparations. Taken together, our findings show that human mast cells express a 
      functional TrkA receptor tyrosine kinase and indicate that NGF may be able to 
      promote certain aspects of mast cell development and/or maturation in humans. Our 
      studies also raise the possibility that human mast cells may represent a 
      potential source for neurotrophins.
FAU - Tam, S Y
AU  - Tam SY
AD  - Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical 
      School, Boston, MA 02215, USA.
FAU - Tsai, M
AU  - Tsai M
FAU - Yamaguchi, M
AU  - Yamaguchi M
FAU - Yano, K
AU  - Yano K
FAU - Butterfield, J H
AU  - Butterfield JH
FAU - Galli, S J
AU  - Galli SJ
LA  - eng
GR  - AI/CA-23990/AI/NIAID NIH HHS/United States
GR  - CA/AI-72074/CA/NCI NIH HHS/United States
GR  - HL-56383/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor, trkA)
SB  - IM
MH  - Cell Line
MH  - Enzyme Activation
MH  - Flow Cytometry
MH  - Humans
MH  - Kinetics
MH  - Mast Cells/*metabolism
MH  - Nerve Growth Factors/*pharmacology
MH  - Proto-Oncogene Proteins/*biosynthesis/genetics
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Receptor Protein-Tyrosine Kinases/*biosynthesis/genetics
MH  - Receptor, trkA
MH  - Receptors, Nerve Growth Factor/*biosynthesis/genetics
EDAT- 1997/09/18 00:00
MHDA- 1997/09/18 00:01
CRDT- 1997/09/18 00:00
PHST- 1997/09/18 00:00 [pubmed]
PHST- 1997/09/18 00:01 [medline]
PHST- 1997/09/18 00:00 [entrez]
AID - S0006-4971(20)58787-9 [pii]
PST - ppublish
SO  - Blood. 1997 Sep 1;90(5):1807-20.