PMID- 9292528
OWN - NLM
STAT- MEDLINE
DCOM- 19970930
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 90
IP  - 5
DP  - 1997 Sep 1
TI  - In vitro negative selection of viral superantigen-reactive thymocytes by thymic 
      dendritic cells.
PG  - 1943-51
AB  - Intrathymic expression of endogenous mouse mammary tumor virus (MMTV)-encoded 
      superantigens (SAg) induces the clonal deletion of T cells bearing SAg-reactive 
      T-cell receptor (TCR) Vbeta elements. However, the identity of the thymic 
      antigen-presenting cells (APC) involved in the induction of SAg tolerance remains 
      to be defined. We have analyzed the potential of dendritic cells (DC) to mediate 
      the clonal deletion of Mtv-7-reactive TCR alphabeta P14 transgenic thymocytes in 
      an in vitro assay. Our results show that both thymic and splenic DC induced the 
      deletion of TCR transgenic double positive (DP) thymocytes. DC appear to be more 
      efficient than splenic B cells as negatively selecting APC in this experimental 
      system. Interestingly, thymic and splenic DC display a differential ability to 
      induce CD4+ SP thymocyte proliferation. These observations suggest that thymic DC 
      may have an important role in the induction of SAg tolerance in vivo.
FAU - Ferrero, I
AU  - Ferrero I
AD  - Department of Cell Biology, Faculty of Biology, Complutense University, Madrid, 
      Spain.
FAU - Anjuere, F
AU  - Anjuere F
FAU - MacDonald, H R
AU  - MacDonald HR
FAU - Ardavin, C
AU  - Ardavin C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
RN  - 0 (Superantigens)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Cell Differentiation/immunology
MH  - Cells, Cultured
MH  - Clone Cells
MH  - Dendritic Cells/*immunology
MH  - Gene Deletion
MH  - Lymphocyte Activation/immunology
MH  - Mammary Tumor Virus, Mouse/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Transgenic
MH  - Receptors, Antigen, T-Cell, alpha-beta/genetics/*immunology
MH  - Superantigens/*immunology
MH  - T-Lymphocytes/*immunology
MH  - Thymus Gland/immunology
EDAT- 1997/09/18 00:00
MHDA- 1997/09/18 00:01
CRDT- 1997/09/18 00:00
PHST- 1997/09/18 00:00 [pubmed]
PHST- 1997/09/18 00:01 [medline]
PHST- 1997/09/18 00:00 [entrez]
AID - S0006-4971(20)58802-2 [pii]
PST - ppublish
SO  - Blood. 1997 Sep 1;90(5):1943-51.