PMID- 9292787
OWN - NLM
STAT- MEDLINE
DCOM- 19971016
LR  - 20131121
IS  - 0315-162X (Print)
IS  - 0315-162X (Linking)
VI  - 24
IP  - 9
DP  - 1997 Sep
TI  - Quercetin, a bioflavonoid, inhibits the induction of interleukin 8 and monocyte 
      chemoattractant protein-1 expression by tumor necrosis factor-alpha in cultured 
      human synovial cells.
PG  - 1680-4
AB  - OBJECTIVE: Tumor necrosis factor (TNF)-alpha is present in synovial fluid of 
      patients with rheumatoid arthritis. It induces the expression of proinflammatory 
      cytokines in synovial cells. Based on our recent finding that reactive oxygen 
      intermediates play important roles in mediating TNF-alpha action, we examined the 
      effect of an antioxidant bioflavonoid, quercetin, on TNF-alpha induced expression 
      of interleukin 8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) in 
      cultured human synovial cells. METHODS: The amounts of mRNA for IL-8 and MCP-1 
      were determined by Northern blot analysis. Electrophoretic mobility shift assays 
      (EMSA) were performed for the detection of a transcription factor, nuclear 
      factor-kappa B (NF-kappa B). RESULTS: Addition of quercetin suppressed TNF-alpha 
      induced increase in the mRNA for IL-8 and MCP-1 in a dose dependent manner. 
      Quercetin did not affect the stability of these mRNA. H2O2 mediated induction of 
      IL-8 and MCP-1 genes was also inhibited by quercetin. EMSA revealed that 
      quercetin inhibited the activation of NF-kappa B by TNF-alpha. CONCLUSION: 
      Quercetin suppresses TNF-alpha mediated stimulation of IL-8 and MCP-1 expression, 
      at least in part, by inhibiting the activation of NF-kappa B.
FAU - Sato, M
AU  - Sato M
AD  - Department of Endocrinology and Metabolism, Nagoya University, Japan.
FAU - Miyazaki, T
AU  - Miyazaki T
FAU - Kambe, F
AU  - Kambe F
FAU - Maeda, K
AU  - Maeda K
FAU - Seo, H
AU  - Seo H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - J Rheumatol
JT  - The Journal of rheumatology
JID - 7501984
RN  - 0 (Chemokine CCL2)
RN  - 0 (DNA Probes)
RN  - 0 (Interleukin-8)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9IKM0I5T1E (Quercetin)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Arthritis, Rheumatoid/drug therapy/metabolism/pathology
MH  - Blotting, Northern
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - DNA Probes/chemistry
MH  - Humans
MH  - Hydrogen Peroxide/antagonists & inhibitors/pharmacology
MH  - Interleukin-8/*biosynthesis/genetics
MH  - NF-kappa B/antagonists & inhibitors
MH  - Quercetin/*pharmacology
MH  - RNA, Messenger/*biosynthesis
MH  - Recombinant Proteins/pharmacology
MH  - Synovial Membrane/drug effects/*metabolism/pathology
MH  - Tumor Necrosis Factor-alpha/*antagonists & inhibitors/pharmacology
EDAT- 1997/09/18 00:00
MHDA- 1997/09/18 00:01
CRDT- 1997/09/18 00:00
PHST- 1997/09/18 00:00 [pubmed]
PHST- 1997/09/18 00:01 [medline]
PHST- 1997/09/18 00:00 [entrez]
PST - ppublish
SO  - J Rheumatol. 1997 Sep;24(9):1680-4.