PMID- 9292803
OWN - NLM
STAT- MEDLINE
DCOM- 19971016
LR  - 20061115
IS  - 0315-162X (Print)
IS  - 0315-162X (Linking)
VI  - 24
IP  - 9
DP  - 1997 Sep
TI  - Suppression of matrix metalloproteinase-3 synthesis by interleukin-4 in human 
      articular chondrocytes.
PG  - 1774-9
AB  - OBJECTIVE: To evaluate the effect of interleukin-4 (IL-4) on IL-1 induced matrix 
      metalloproteinase-3 (MMP-3) and tissue inhibitor of metalloproteinase-1 (TIMP-1) 
      production by human articular chondrocytes. METHODS: Monolayer cell culture of 
      chondrocytes was obtained from human articular cartilage from patella within 24 h 
      after death. MMP-3 and TIMP-1 protein levels were determined by ELISA. MMP-3 
      activity was assayed as caseinase activity. Amounts of MMP-3 and TIMP-1 mRNA were 
      measured by Northern blot analysis. RESULTS: IL-4 suppressed IL-1 stimulated 
      MMP-3 protein and enzyme activity. Moreover, IL-4 suppressed IL-1 induced MMP-3 
      mRNA. In contrast, IL-4 did not alter the level of TIMP-1 protein and mRNA. 
      CONCLUSION: IL-4 may be implicated as a protective mediator of joint destruction 
      seen in inflammatory arthritis.
FAU - Nemoto, O
AU  - Nemoto O
AD  - Department of Orthopaedic Surgery, National Defense Medical College, Saitama, 
      Japan.
FAU - Yamada, H
AU  - Yamada H
FAU - Kikuchi, T
AU  - Kikuchi T
FAU - Shinmei, M
AU  - Shinmei M
FAU - Obata, K
AU  - Obata K
FAU - Sato, H
AU  - Sato H
FAU - Seiki, M
AU  - Seiki M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - J Rheumatol
JT  - The Journal of rheumatology
JID - 7501984
RN  - 0 (Glycoproteins)
RN  - 0 (Growth Substances)
RN  - 0 (Interleukin-1)
RN  - 0 (Protease Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - 207137-56-2 (Interleukin-4)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.- (caseinase)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
EIN - J Rheumatol 1998 Jan;25(1):193. Shimmei M [corrected to Shinmei M]
MH  - Adult
MH  - Cartilage, Articular/*drug effects/*enzymology
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Glycoproteins/genetics/metabolism
MH  - Growth Substances/pharmacology
MH  - Humans
MH  - Interleukin-1/antagonists & inhibitors/pharmacology
MH  - Interleukin-4/*pharmacology
MH  - Matrix Metalloproteinase 3/genetics/*metabolism
MH  - Metalloendopeptidases/metabolism
MH  - Protease Inhibitors/metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Tissue Inhibitor of Metalloproteinases
EDAT- 1997/09/18 00:00
MHDA- 1997/09/18 00:01
CRDT- 1997/09/18 00:00
PHST- 1997/09/18 00:00 [pubmed]
PHST- 1997/09/18 00:01 [medline]
PHST- 1997/09/18 00:00 [entrez]
PST - ppublish
SO  - J Rheumatol. 1997 Sep;24(9):1774-9.