PMID- 9294024
OWN - NLM
STAT- MEDLINE
DCOM- 19971008
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 392
IP  - 3
DP  - 1997 Aug 14
TI  - Evaluation of the rat bone marrow and peripheral blood micronucleus test using 
      monocrotaline.
PG  - 243-9
AB  - The present study was performed as part of the 9th collaborative trial of the 
      CSGMT (Collaborative Study Group for the Micronucleus Test) to evaluate the 
      performance of the rat micronucleus test in detection of clastogenic agents. 
      Temporary fluorescent staining using acridine orange (AO) was compared with 
      permanent staining using a modified Feulgen technique for bone marrow and Giemsa 
      for blood. The Feulgen and AO methods were both found to be suitable for 
      demonstrating micronucleus induction in rat bone marrow following oral 
      administration of monocrotaline and cyclophosphamide. Induction of micronuclei 
      was also shown using rat blood. The simple AO supravital method described 
      produced uniformly stained preparations which were extremely easy to assess and 
      interpret. The AO method was preferred to Giemsa for analysis of blood, but it 
      was essential to analyse samples within 4 days of collection to avoid excessive 
      deterioration of cells. Because of the relatively low number of micronucleated 
      cells found in rat blood, it is suggested that examination of a larger number of 
      cells may be necessary to achieve a similar sensitivity to the bone marrow assay 
      for routine screening.
FAU - Proudlock, R J
AU  - Proudlock RJ
AD  - Department of Cellular Toxicology, Huntingdon Life Sciences, Cambridgeshire, UK. 
      ProudloR@UKOrg.Huntingdon.com
FAU - Statham, J
AU  - Statham J
FAU - Howard, W
AU  - Howard W
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Azure Stains)
RN  - 0 (Coloring Agents)
RN  - 0 (Feulgen stain)
RN  - 0 (Mutagens)
RN  - 0 (Rosaniline Dyes)
RN  - 73077K8HYV (Monocrotaline)
RN  - 8N3DW7272P (Cyclophosphamide)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Azure Stains
MH  - Bone Marrow/drug effects
MH  - Bone Marrow Cells
MH  - Coloring Agents
MH  - Cyclophosphamide/toxicity
MH  - Erythrocytes/drug effects
MH  - Evaluation Studies as Topic
MH  - Female
MH  - Male
MH  - Micronucleus Tests/*methods
MH  - Monocrotaline/*toxicity
MH  - Mutagens/*toxicity
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Rosaniline Dyes
MH  - Sensitivity and Specificity
EDAT- 1997/08/14 00:00
MHDA- 1997/09/18 00:01
CRDT- 1997/08/14 00:00
PHST- 1997/08/14 00:00 [pubmed]
PHST- 1997/09/18 00:01 [medline]
PHST- 1997/08/14 00:00 [entrez]
AID - S1383-5718(97)00077-6 [pii]
AID - 10.1016/s1383-5718(97)00077-6 [doi]
PST - ppublish
SO  - Mutat Res. 1997 Aug 14;392(3):243-9. doi: 10.1016/s1383-5718(97)00077-6.