PMID- 9294847
OWN - NLM
STAT- MEDLINE
DCOM- 19980108
LR  - 20190512
IS  - 1360-9947 (Print)
IS  - 1360-9947 (Linking)
VI  - 3
IP  - 8
DP  - 1997 Aug
TI  - The detection and confirmation of 11 beta-hydroxysteroid dehydrogenase type 1 
      transcripts in human luteinized granulosa cells using RT-PCR and plasmid pUC18.
PG  - 651-4
AB  - The activity of 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD) in human 
      granulosa cells has been shown to be associated with the outcome of treatment 
      following in-vitro fertilization and embryo transfer. There are two known 
      isoforms of 11 beta-HSD which differ significantly in their actions and co-factor 
      requirements. The net activity of 11 beta-HSD which differ significantly in their 
      actions and co-factor requirements. The net activity of 11 beta-HSD within the 
      human ovary is unclear, but may be of particular importance within the ovarian 
      follicle in regulating possible glucocorticoid influences on the oocyte. This 
      study presents preliminary information regarding establishment of techniques to 
      identify transcripts of the 11 beta-HSD isoforms within human granulosa cells and 
      human cumulus cells using reverse transcription-polymerase chain reaction. In 
      view of the high expression of the type 1 11 beta-HSD isoform and the possibility 
      of other 11 beta-HSD isoforms in the ovary, plasmid technology was used to 
      confirm the technique specifically identifying the known isoforms.
FAU - Smith, M P
AU  - Smith MP
AD  - University of Bristol, Division of Obstetrics and Gynaecology, St. Michael's 
      Hospital, UK.
FAU - Keay, S D
AU  - Keay SD
FAU - Hall, L
AU  - Hall L
FAU - Harlow, C R
AU  - Harlow CR
FAU - Jenkins, J M
AU  - Jenkins JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mol Hum Reprod
JT  - Molecular human reproduction
JID - 9513710
RN  - 0 (Isoenzymes)
RN  - 0 (Recombinant Proteins)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - Cloning, Organism
MH  - Corpus Luteum/*physiology
MH  - Female
MH  - Granulosa Cells/*enzymology
MH  - Humans
MH  - Hydroxysteroid Dehydrogenases/*biosynthesis
MH  - Isoenzymes/*biosynthesis
MH  - Luteal Cells/enzymology
MH  - Oocytes/physiology
MH  - *Plasmids
MH  - Polymerase Chain Reaction/methods
MH  - Recombinant Proteins/biosynthesis
MH  - *Transcription, Genetic
EDAT- 1997/08/01 00:00
MHDA- 1997/09/19 00:01
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 1997/09/19 00:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
AID - 10.1093/molehr/3.8.651 [doi]
PST - ppublish
SO  - Mol Hum Reprod. 1997 Aug;3(8):651-4. doi: 10.1093/molehr/3.8.651.