PMID- 9295217
OWN - NLM
STAT- MEDLINE
DCOM- 19971023
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 764
IP  - 1-2
DP  - 1997 Aug 1
TI  - Pretreatment with the protein kinase C activator phorbol 12,13-dibutyrate 
      attenuates the ethanol-induced loss of the righting reflex in mice: modification 
      by diabetes.
PG  - 244-8
AB  - The effects of the protein kinase C (PKC) activator phorbol 12,13-dibutyrate 
      (PDBu) on the ethanol-induced loss of the righting reflex were studied in 
      diabetic and non-diabetic mice. The ethanol-induced loss of the righting reflex 
      was significantly less in diabetic mice than in non-diabetic mice. 
      Intracerebroventricular (i.c.v.) pretreatment with PDBu dose- and 
      time-dependently reduced the ethanol-induced loss of the righting reflex in 
      non-diabetic mice. The reduction of the ethanol-induced loss of the righting 
      reflex caused by PDBu was reversed by concomitant i.c.v. pretreatment with 
      calphostin C, a selective PKC inhibitor. On the other hand, PDBu had no effect on 
      the ethanol-induced loss of the righting reflex in diabetic mice. I.c.v. 
      pretreatment with calphostin C (10 pmol) increased the ethanol-induced loss of 
      the righting reflex in diabetic mice but not in non-diabetic mice. These results 
      suggest that the activation of PKC reduces the ethanol-induced loss of the 
      righting reflex in mice. Furthermore, it is possible that this attenuation of the 
      ethanol-induced loss of the righting reflex in diabetic mice may be due in part 
      to increased PKC activity.
FAU - Ohsawa, M
AU  - Ohsawa M
AD  - Department of Pathophysiology and Therapeutics, Faculty of Pharmaceutical 
      Sciences, Hoshi University, Tokyo, Japan.
FAU - Kamei, J
AU  - Kamei J
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Central Nervous System Depressants)
RN  - 37558-16-0 (Phorbol 12,13-Dibutyrate)
RN  - 3K9958V90M (Ethanol)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Animals
MH  - Central Nervous System Depressants/*pharmacology
MH  - Diabetes Mellitus, Experimental/*physiopathology
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Activation/drug effects
MH  - Ethanol/*pharmacology
MH  - Injections, Intraventricular
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Phorbol 12,13-Dibutyrate/administration & dosage/*pharmacology
MH  - Postural Balance/*drug effects
MH  - Protein Kinase C/*metabolism
EDAT- 1997/08/01 00:00
MHDA- 1997/09/19 00:01
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 1997/09/19 00:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
AID - S0006-8993(97)00601-X [pii]
AID - 10.1016/s0006-8993(97)00601-x [doi]
PST - ppublish
SO  - Brain Res. 1997 Aug 1;764(1-2):244-8. doi: 10.1016/s0006-8993(97)00601-x.