PMID- 9298128
OWN - NLM
STAT- MEDLINE
DCOM- 19980114
LR  - 20190201
IS  - 0001-5555 (Print)
IS  - 0001-5555 (Linking)
VI  - 77
IP  - 5
DP  - 1997 Sep
TI  - Phorbol-myristate-acetate, but not interleukin-1 beta or insulin-like growth 
      factor-I, regulates protein kinase C isoenzymes in human dermal papilla cells.
PG  - 361-4
AB  - The in vitro growth of human hair follicles is inhibited by interleukin (IL)-1 
      beta and phorbol esters, such as phorbol-myristate-acetate (PMA), but enhanced by 
      insulin-like growth factor (IGF)-I. Although this process is only incompletely 
      understood, the dermal papilla as a pivotal part of the hair follicle is almost 
      certainly involved. Since protein kinase C (PKC) isoenzymes are activated by 
      phorbol esters and are key enzymes in signalling pathways of several hormones, 
      neurotransmitters, and growth factors, we addressed the question whether the 
      action of the above-mentioned hair growth-modulating substances may affect PKC 
      isoenzymes in cultured dermal papilla cells (DPC). By Western blot analysis, 
      protein kinase C alpha, -epsilon, -gamma, -iota, -lambda, and the RACK1 receptor 
      protein were detected in dermal papilla cell cultures, whereas the isoenzymes 
      delta and mu were expressed only at low levels and protein kinase C-beta, -theta, 
      and -zeta, were not present. After PMA stimulation, the PKC alpha, -epsilon, and 
      -gamma were translocated from the cytosol to the membrane fraction and 
      subsequently down-regulated. PKC iota was down-regulated but not translocated, 
      and PKC lambda and RACK1 were not affected by PMA. Neither, IL-1 beta nor IGF had 
      an effect on PKC or RACK1 expression. We conclude that cultured DPC express a 
      distinct PKC isoenzyme pattern and that the PMA-induced growth arrest in 
      cultivated hair follicles may be transmitted via protein kinases, whereas the 
      effects of IL-1 beta or IGF may be transduced via other signal transduction 
      pathways or other cell types.
FAU - Eicheler, W
AU  - Eicheler W
AD  - Department of Dermatology, Philipp University, Marburg, Germany. 
      eicheler@mailer.unimarburg.de
FAU - Huth, A
AU  - Huth A
FAU - Happle, R
AU  - Happle R
FAU - Hoffmann, R
AU  - Hoffmann R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Sweden
TA  - Acta Derm Venereol
JT  - Acta dermato-venereologica
JID - 0370310
RN  - 0 (Interleukin-1)
RN  - 0 (Isoenzymes)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Hair Follicle/drug effects/*enzymology/growth & development
MH  - Humans
MH  - Immunohistochemistry
MH  - In Vitro Techniques
MH  - Insulin-Like Growth Factor I/*metabolism/pharmacology
MH  - Interleukin-1/*metabolism/pharmacology
MH  - Isoenzymes/drug effects/*metabolism
MH  - Protein Kinase C/drug effects/*metabolism
MH  - Reference Values
MH  - Signal Transduction/drug effects/physiology
MH  - Tetradecanoylphorbol Acetate/*metabolism/pharmacology
EDAT- 1997/09/23 00:00
MHDA- 1997/09/23 00:01
CRDT- 1997/09/23 00:00
PHST- 1997/09/23 00:00 [pubmed]
PHST- 1997/09/23 00:01 [medline]
PHST- 1997/09/23 00:00 [entrez]
AID - 10.2340/0001555577361364 [doi]
PST - ppublish
SO  - Acta Derm Venereol. 1997 Sep;77(5):361-4. doi: 10.2340/0001555577361364.