PMID- 9299617
OWN - NLM
STAT- MEDLINE
DCOM- 19971009
LR  - 20081121
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 236
IP  - 1
DP  - 1997 Sep 15
TI  - Strain-specific expression of spliced MMTV RNAs containing the superantigen gene.
PG  - 54-65
AB  - The transmission of milk-borne or exogenous mouse mammary tumor virus (MMTV) 
      requires infection of B cells in the gut-associated lymphoid tissue and 
      expression of the superantigen (Sag) protein at the B-cell surface. Presentation 
      of Sag at the B-cell surface is required for the transmission of MMTV to T cells 
      and subsequent infection of the target mammary gland tissue. Because several 
      different promoters have been reported for MMTV sag mRNA expression, we 
      investigated whether the detection of spliced sag RNAs was dependent upon the 
      cell type infected or the particular MMTV strain examined. In this study, we 
      detected expression of spliced sag RNA from the standard promoter and from an 
      internal U3 promoter in B-cell lines expressing endogenous Mtv-6 by RT-PCR, 
      although expression from the standard promoter appeared to be at least 10-fold 
      higher than that observed from the internal U3 promoter. Sag RNA originating from 
      exogenous C3H MMTV was not observed from either of the U3 promoters in any cell 
      type examined. However, spliced mRNAs containing the exogenous C3H MMTV, 
      endogenous Mtv-8, or endogenous Mtv-17 sag genes could be detected from a 
      previously described promoter in the envelope coding region regardless of the 
      cell type infected. Because sag-specific RNAs can be initiated independently of 
      the LTR promoters, there may be selection for independent control of MMTV sag and 
      structural gene expression.
CI  - Copyright 1997 Academic Press.
FAU - Xu, L
AU  - Xu L
AD  - Department of Microbiology, The University of Texas at Austin, Austin, Texas 
      78712, USA.
FAU - Wrona, T J
AU  - Wrona TJ
FAU - Dudley, J P
AU  - Dudley JP
LA  - eng
GR  - CA34780/CA/NCI NIH HHS/United States
GR  - CA52646/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antigens, Viral)
RN  - 0 (RNA, Viral)
RN  - 0 (Superantigens)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Viral/biosynthesis/genetics
MH  - B-Lymphocytes/immunology/virology
MH  - Base Sequence
MH  - Consensus Sequence
MH  - Exons
MH  - Genes, Viral
MH  - Genes, env
MH  - Genes, pol
MH  - Introns
MH  - Lymphoma, B-Cell
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C3H
MH  - Mice, Inbred Strains
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Promoter Regions, Genetic
MH  - Proviruses/genetics/physiology
MH  - *RNA Splicing
MH  - RNA, Viral/*biosynthesis
MH  - Sequence Alignment
MH  - Superantigens/*biosynthesis/genetics
MH  - T-Lymphocytes/immunology/virology
MH  - *Transcription, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1997/09/23 00:00
MHDA- 1997/09/23 00:01
CRDT- 1997/09/23 00:00
PHST- 1997/09/23 00:00 [pubmed]
PHST- 1997/09/23 00:01 [medline]
PHST- 1997/09/23 00:00 [entrez]
AID - S0042-6822(97)98717-1 [pii]
AID - 10.1006/viro.1997.8717 [doi]
PST - ppublish
SO  - Virology. 1997 Sep 15;236(1):54-65. doi: 10.1006/viro.1997.8717.