PMID- 9301637
OWN - NLM
STAT- MEDLINE
DCOM- 19970930
LR  - 20190831
IS  - 1079-5642 (Print)
IS  - 1079-5642 (Linking)
VI  - 17
IP  - 8
DP  - 1997 Aug
TI  - Induction of the acute-phase reaction increases heparin-binding proteins in 
      plasma.
PG  - 1568-74
AB  - We have previously demonstrated that the nonspecific binding of unfractionated 
      heparin (UFH) to plasma proteins has a marked modulating effect on its 
      anticoagulant activity. Since some heparin-binding proteins are also 
      acute-phase-reactant proteins, we explored the possibility that the induction of 
      the acute-phase response can increase the plasma concentrations of 
      heparin-binding proteins. The recovery of a fixed amount of UFH or 
      low-molecular-weight heparin (LMWH) added in vitro to rat plasma samples obtained 
      at various time intervals after the administration of intravenous endotoxin or 
      subcutaneous turpentine was compared with that of saline-treated control animals. 
      The anti-factor Xa activity was measured in the plasma samples before and after 
      the addition of a chemically modified low-affinity heparin (LAH) to displace the 
      proportion of the added heparin that is reversibly bound to plasma proteins. Our 
      results show that at 6 hours post-endotoxin and at 24 hours post-turpentine 
      treatment, virtually no anti-factor Xa activity could be measured in the plasma 
      samples, while the expected levels were obtained for control plasma. After the 
      addition of LAH to displace protein-bound UFH, essentially the same anti-factor 
      Xa levels were measured in the plasma from all three treatment groups. These 
      results indicate that induction of the acute-phase reaction can dramatically 
      increase the levels of heparin-binding proteins in rat plasma. In addition, we 
      compared the anti-factor Xa recovery of UFH with that of an LMWH from the plasma 
      of endotoxin- and saline-treated rats and demonstrated that LMWH binds less to 
      plasma proteins than UFH, even in plasma in which the levels of heparin-binding 
      proteins are markedly elevated. The recovery of a fixed amount of UFH added in 
      vitro to human plasma from septic patients was also reduced, but not to the same 
      extent as seen in rat plasma. Removal of candidate heparin-binding and 
      acute-phase proteins by immunodepletion indicated that vitronectin plays an 
      important role in the nonspecific binding of UFH in patient plasma.
FAU - Young, E
AU  - Young E
AD  - Department of Pathology, McMaster University, Hamilton, Ontario, Canada.
FAU - Podor, T J
AU  - Podor TJ
FAU - Venner, T
AU  - Venner T
FAU - Hirsh, J
AU  - Hirsh J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arterioscler Thromb Vasc Biol
JT  - Arteriosclerosis, thrombosis, and vascular biology
JID - 9505803
RN  - 0 (Carrier Proteins)
RN  - 0 (Endotoxins)
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 0 (Vitronectin)
RN  - 9005-49-6 (Heparin)
RN  - XJ6RUH0O4G (Turpentine)
SB  - IM
MH  - Acute-Phase Reaction/*blood/*etiology
MH  - Animals
MH  - Blotting, Western
MH  - Carrier Proteins/*blood
MH  - Endotoxins/pharmacology
MH  - Heparin/*blood
MH  - Heparin, Low-Molecular-Weight/blood
MH  - Humans
MH  - Lymphocyte Depletion
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sepsis/blood
MH  - Turpentine/pharmacology
MH  - Vitronectin/blood
EDAT- 1997/08/01 00:00
MHDA- 1997/09/25 00:01
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 1997/09/25 00:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
AID - 10.1161/01.atv.17.8.1568 [doi]
PST - ppublish
SO  - Arterioscler Thromb Vasc Biol. 1997 Aug;17(8):1568-74. doi: 
      10.1161/01.atv.17.8.1568.