PMID- 9303071
OWN - NLM
STAT- MEDLINE
DCOM- 19971016
LR  - 20061115
IS  - 1065-7541 (Print)
IS  - 1065-7541 (Linking)
VI  - 5
IP  - 3
DP  - 1997
TI  - Initiation of oncogenic transformation in human mammary epithelial cells by 
      charged particles.
PG  - 134-8
AB  - Experimental studies have shown that high linear-energy transfer (LET) charged 
      particles can be more effective than x-rays and gamma-rays in inducing oncogenic 
      transformation in cultured cells and tumors in animals. Based on these results, 
      experiments were designed and performed with an immortal human mammary epithelial 
      cell line (H184B5), and several clones transformed by heavy ions were obtained. 
      Cell fusion experiments were subsequently done, and results indicate that the 
      transforming gene(s) is recessive. Chromosome analysis with fluorescence in situ 
      hybridization (FISH) techniques also showed additional translocations in 
      transformed human mammary epithelial cells. In addition, studies with these cell 
      lines indicate that heavy ions can effectively induce deletion, break, and 
      dicentrics. Deletion of tumor suppressor gene(s) and/or formation of 
      translocation through DNA double strand breaks is a likely mechanism for the 
      initiation of oncogenic transformation in human mammary epithelial cells.
FAU - Yang, T C
AU  - Yang TC
AD  - Radiation Biophysics Laboratory, NASA Johnson Space Center, Houston, Texas 77058, 
      USA. tyang@plato.jsc.nasa.gov
FAU - Georgy, K A
AU  - Georgy KA
FAU - Craise, L M
AU  - Craise LM
FAU - Durante, M
AU  - Durante M
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Radiat Oncol Investig
JT  - Radiation oncology investigations
JID - 9437448
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Breast/pathology/*radiation effects
MH  - Cell Fusion
MH  - Cell Line
MH  - Cell Transformation, Neoplastic/pathology/*radiation effects
MH  - Cells, Cultured
MH  - Chromosome Breakage
MH  - Chromosome Deletion
MH  - Chromosomes/chemistry/genetics
MH  - DNA/genetics/radiation effects
MH  - DNA Damage
MH  - Epithelium/pathology/radiation effects
MH  - Female
MH  - Gamma Rays
MH  - Gene Deletion
MH  - Genes, Recessive/genetics
MH  - Genes, Tumor Suppressor/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Linear Energy Transfer
MH  - Neoplasms, Radiation-Induced/etiology
MH  - *Radiation, Ionizing
MH  - Transformation, Genetic/genetics
MH  - Translocation, Genetic/genetics
MH  - X-Rays
OTO - NASA
OT  - NASA Center JSC
OT  - NASA Discipline Radiation Health
FIR - Yang, T C
IR  - Yang TC
IRAD- JSC
EDAT- 1997/01/01 00:00
MHDA- 1997/09/26 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/09/26 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1002/(SICI)1520-6823(1997)5:3<134::AID-ROI10>3.0.CO;2-6 [doi]
PST - ppublish
SO  - Radiat Oncol Investig. 1997;5(3):134-8. doi: 
      10.1002/(SICI)1520-6823(1997)5:3<134::AID-ROI10>3.0.CO;2-6.