PMID- 9303484
OWN - NLM
STAT- MEDLINE
DCOM- 19971008
LR  - 20041117
IS  - 0270-9139 (Print)
IS  - 0270-9139 (Linking)
VI  - 26
IP  - 3
DP  - 1997 Sep
TI  - Antibodies to single-stranded and double-stranded DNA in antinuclear 
      antibody-positive type 1-autoimmune hepatitis.
PG  - 567-72
AB  - To determine the significance of antibodies to single-stranded (anti-ssDNA) and 
      double-stranded DNA (anti-dsDNA) in antinuclear antibody (ANA)-positive type 1 
      autoimmune hepatitis, sera from 53 patients were tested by enzyme immunosorbent 
      assay (ELISA) and indirect immunofluorescence using the Crithidia luciliae 
      substrate. Anti-dsDNA were detected in 18 patients (34%) by ELISA and 12 patients 
      (23%) by the Crithidia-based assay. Twenty patients with anti-dsDNA by either 
      assay (38%) had higher serum levels of immunoglobulin G (3971 +/- 270 mg/dL vs. 
      3201 +/- 247 mg/dL, P = .05) than seronegative patients. They also had human 
      leukocyte antigen (HLA) DR4 more commonly than other patients (83% vs. 41%, P = 
      .006) and normal subjects (83% vs. 30%, P = .00007). In contrast to patients 
      seropositive by the Crithidia-based assay, those seropositive by ELISA failed 
      corticosteroid therapy more commonly (24% vs. 3%, P = .04). Anti-ssDNA were found 
      in 45 patients (85%) and they did not distinguish patients with different 
      clinical features or outcomes. We conclude that anti-dsDNA are common in 
      ANA-positive type 1 autoimmune hepatitis. HLA DR4 is associated with their 
      production, and seropositivity by ELISA characterizes patients who have a poorer 
      immediate response to corticosteroid treatment. Anti-ssDNA are common but they do 
      not have important clinical implications.
FAU - Czaja, A J
AU  - Czaja AJ
AD  - Division of Gastroenterology and Internal Medicine, Mayo Clinic and Mayo 
      Foundation, Rochester, MN 55905, USA.
FAU - Morshed, S A
AU  - Morshed SA
FAU - Parveen, S
AU  - Parveen S
FAU - Nishioka, M
AU  - Nishioka M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Hepatology
JT  - Hepatology (Baltimore, Md.)
JID - 8302946
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (HLA-DR Antigens)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Antibodies, Antinuclear/*blood
MH  - Autoimmune Diseases/*immunology/physiopathology/therapy
MH  - Crithidia
MH  - DNA/*immunology
MH  - DNA, Single-Stranded/*immunology
MH  - Disease Progression
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Fluorescent Antibody Technique, Indirect
MH  - HLA-DR Antigens/blood
MH  - Hepatitis/*immunology/physiopathology/therapy
MH  - Humans
MH  - Liver/pathology
MH  - Liver Function Tests
MH  - Male
MH  - Middle Aged
MH  - Physical Examination
MH  - Recurrence
MH  - Treatment Outcome
EDAT- 1997/09/26 00:00
MHDA- 1997/09/26 00:01
CRDT- 1997/09/26 00:00
PHST- 1997/09/26 00:00 [pubmed]
PHST- 1997/09/26 00:01 [medline]
PHST- 1997/09/26 00:00 [entrez]
AID - S0270913997003844 [pii]
AID - 10.1002/hep.510260306 [doi]
PST - ppublish
SO  - Hepatology. 1997 Sep;26(3):567-72. doi: 10.1002/hep.510260306.