PMID- 9305602
OWN - NLM
STAT- MEDLINE
DCOM- 19971016
LR  - 20190914
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 11
IP  - 9
DP  - 1997 Sep
TI  - BCR/ABL-negative progenitors are enriched in the adherent fraction of CD34+ cells 
      circulating in the blood of chronic phase chronic myeloid leukemia patients.
PG  - 1486-92
AB  - Philadelphia chromosome-positive (Ph+) hemopoietic cells predominate in patients 
      with chronic myeloid leukemia (CML) in chronic phase, but some Ph presumably 
      normal stem cells persist in most patients. Ph cells are relatively frequent, 
      compared to mature cell populations, in primitive hemopoietic cell populations 
      from CML patients. We have purified CD34+ cells from chronic phase CML blood and 
      separated them into two fractions on the basis of adherence or non-adherence to 
      tissue culture plastic. We also separated CD34+ CML cell populations into 
      HLA-DR(hi) and HLA-DR(lo) fractions and CD38(hi) and CD38(lo) fractions by flow 
      cytometry. The CD34+ cells that adhered to plastic were predominantly CD33-, 
      CD38- and HLA(-)-DR; cells with these phenotypic properties were significantly 
      rarer in the CD34+ non-adherent cell population (P = 0.008-0.02). Expression of 
      p210 BCR/ABL mRNA by adherent, non-adherent, HLA-DR(hi) and HLA-DR(lo)CD34+ cell 
      subpopulations was demonstrated by RT-PCR. Using fluorescence in situ 
      hybridization (FISH) in conjunction with BCR and ABL probes we detected Ph+ and 
      Ph- cells in both adherent and non-adherent CD34+ cell fractions of 15/15 
      patients studied and in the HLA-DR(lo) or CD38(lo) sorted CD34+ cell fractions. 
      The concentration of Ph- cells in the adherent CD34+ cell fraction was three-fold 
      higher than in the non-adherent fraction (P = 0.001). Ph- adherent cells were 
      detected in untreated CML patients and as late as 6 years after diagnosis of CML 
      in patients treated with hydroxyurea (HU) or interferon-alpha (IFN-alpha). We 
      conclude that whilst appreciable numbers of Ph- primitive hemopoietic progenitors 
      are present in the circulation in untreated patients and also in treated patients 
      in late chronic phase, the majority of cells expressing CD34 but not CD33, CD38 
      or HLA-DR antigens, are part of the CML clone.
FAU - Grand, F H
AU  - Grand FH
AD  - Leukemaemia Research Fund Centre for Adult Leukaemia, Royal Postgraduate Medical 
      School, London, UK.
FAU - Marley, S B
AU  - Marley SB
FAU - Chase, A
AU  - Chase A
FAU - Titley, I
AU  - Titley I
FAU - Healy, L
AU  - Healy L
FAU - Spencer, A
AU  - Spencer A
FAU - Reiter, A
AU  - Reiter A
FAU - Goldman, J M
AU  - Goldman JM
FAU - Gordon, M Y
AU  - Gordon MY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (Plastics)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adult
MH  - Bone Marrow/pathology
MH  - Cell Adhesion
MH  - Cell Separation/methods
MH  - Chromosomes, Human, Pair 22
MH  - Chromosomes, Human, Pair 9
MH  - Female
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Hematopoietic Stem Cells/*pathology
MH  - Humans
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/blood/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Plastics
MH  - RNA, Messenger/genetics
MH  - RNA, Neoplasm/genetics
MH  - Translocation, Genetic
EDAT- 1997/09/26 00:00
MHDA- 1997/09/26 00:01
CRDT- 1997/09/26 00:00
PHST- 1997/09/26 00:00 [pubmed]
PHST- 1997/09/26 00:01 [medline]
PHST- 1997/09/26 00:00 [entrez]
AID - 10.1038/sj.leu.2400748 [doi]
PST - ppublish
SO  - Leukemia. 1997 Sep;11(9):1486-92. doi: 10.1038/sj.leu.2400748.