PMID- 9307844
OWN - NLM
STAT- MEDLINE
DCOM- 19971029
LR  - 20061115
IS  - 0361-090X (Print)
IS  - 0361-090X (Linking)
VI  - 21
IP  - 5
DP  - 1997
TI  - Lack of correlation between codon 12 K-ras mutations and major histocompatibility 
      complex antigens in bronchogenic carcinomas.
PG  - 412-7
AB  - In experimental systems, an association between K-ras activity and expression of 
      major histocompatibility complex (MHC) molecules has been reported. In this 
      study, 52 surgically resected bronchogenic carcinomas were studied for human 
      leukocyte antigen (HLA) class I and II expression, and for the presence of point 
      mutations in codon 12 of the K-ras gene. HLA class I loss was detected in 18 
      carcinomas, and most of the tumors (43 cases) were found negative for HLA class 
      II antigen expression by the APAAP technique with specific monoclonal antibodies. 
      Analysis using the polymerase chain reaction (PCR), together with selective 
      hybridization using mutation-specific synthetic oligonucleotides, demonstrated 
      K-ras mutations in five cases, all of them corresponding to the adenocarcinoma 
      subtype (31.2% of the adenocarcinomas included in our study) with a poor degree 
      of differentiation. We did not find any correlation between K-ras mutations and 
      HLA class I and II expression in bronchogenic carcinomas. Therefore, it would 
      appear that downregulation of MHC antigens by point mutations of K-ras does not 
      take place in vivo.
FAU - Redondo, M
AU  - Redondo M
AD  - Department of Biochemistry, Costa del Sol Hospital, Marbella, Malaga, Spain.
FAU - Rodriguez, F
AU  - Rodriguez F
FAU - Hortas, M L
AU  - Hortas ML
FAU - Concha, A
AU  - Concha A
FAU - Morell, M
AU  - Morell M
FAU - Garrido, F
AU  - Garrido F
FAU - Ruiz-Cabello, F
AU  - Ruiz-Cabello F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cancer Detect Prev
JT  - Cancer detection and prevention
JID - 7704778
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Carcinoma, Bronchogenic/*genetics
MH  - Genes, ras/*genetics
MH  - Histocompatibility Antigens Class I/*analysis
MH  - Histocompatibility Antigens Class II/*analysis
MH  - Humans
MH  - Lung Neoplasms/*genetics
MH  - Point Mutation/*genetics
EDAT- 1997/01/01 00:00
MHDA- 1997/10/06 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/10/06 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
PST - ppublish
SO  - Cancer Detect Prev. 1997;21(5):412-7.