PMID- 9308027
OWN - NLM
STAT- MEDLINE
DCOM- 19971121
LR  - 20190914
IS  - 0143-4179 (Print)
IS  - 0143-4179 (Linking)
VI  - 31
IP  - 4
DP  - 1997 Aug
TI  - Further evidence for the involvement of tachykinin receptor subtypes in formalin 
      and capsaicin models of pain in mice.
PG  - 381-9
AB  - The intradermal (i.d.) injection of NK1 receptor antagonists GR 82334 and FK 888 
      (1-50 pmol/paw), in association with formalin, produced graded inhibition of the 
      early but not the late phase of the formalin test. The NK2, SR 48968 and NK3 SR 
      142801 receptor antagonists (1-50 pmol/paw) were effective in inhibiting both 
      phases of the formalin model. Co-injection of NK1, (FK 888, GR 82334), NK2 (SR 
      48968) or NK3 (SR 142801) receptor antagonists with capsaicin dose-dependently 
      attenuated capsaicin-induced licking. In addition, all antagonists were more 
      efficacious when compared with response in the formalin test. The antinociception 
      caused by i.d. injection of the NK3 receptor antagonist SR 142801 against both 
      phases of the formalin test, but not that of NK1 and NK2 receptor antagonists, 
      was significantly reversed by intraperitoneal (i.p.) injection of naloxone (5 
      mg/kg). Intracerebroventricular (i.c.v.) injection of NK1, NK2 or NK3 receptor 
      antagonists (15-500 pmol/site), all produced significant and dose-dependent 
      inhibition of both phases of the formalin and capsaicin tests. With the exception 
      of the response of SR 48968, which was equipotent in both models of nociception, 
      FK 888, GR 82334 and SR 142801 were about 2-25-fold less potent at the ID50 level 
      against the capsaicin-induced pain. The antinociception caused by i.c.v. 
      injection of NK1, NK2 or NK3 receptor antagonists was reversed by i.p. injection 
      of naloxone (5 mg/kg). These results indicate that tachykinin receptor 
      antagonists, acting through NK1, NK2 and NK3 receptors, produce powerful 
      antinociception when injected i.d. or by i.c.v. route against both formalin- and 
      capsaicin-induced licking, being more efficacious against the latter model of 
      nociception. The action of NK3 receptor antagonist given i.d. was mediated 
      through an opioid mechanism sensitive to naloxone. However, when injected i.c.v., 
      the antinociception caused by NK1, NK2 or NK3 receptor antagonists was largely 
      reversed by naloxone when assessed in the formalin test, suggesting a distinct 
      mechanism of action.
FAU - Santos, A R
AU  - Santos AR
AD  - Department of Pharmacology, CCB, Universidade Federal de Santa Catarina, 
      Florianopolis-SC, Brazil.
FAU - Calixto, J B
AU  - Calixto JB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Neuropeptides
JT  - Neuropeptides
JID - 8103156
RN  - 0 (Receptors, Tachykinin)
RN  - 1HG84L3525 (Formaldehyde)
RN  - S07O44R1ZM (Capsaicin)
SB  - IM
MH  - Animals
MH  - Behavior, Animal/drug effects
MH  - Capsaicin/administration & dosage/*pharmacology
MH  - Disease Models, Animal
MH  - Drug Interactions
MH  - Formaldehyde/administration & dosage/*pharmacology
MH  - Injections, Intradermal
MH  - Injections, Intraperitoneal
MH  - Injections, Intraventricular
MH  - Male
MH  - Mice
MH  - Pain/chemically induced/*metabolism
MH  - Receptors, Tachykinin/antagonists & inhibitors/*drug effects/*physiology
EDAT- 1997/08/01 00:00
MHDA- 1997/10/06 00:01
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 1997/10/06 00:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
AID - S0143-4179(97)90075-5 [pii]
AID - 10.1016/s0143-4179(97)90075-5 [doi]
PST - ppublish
SO  - Neuropeptides. 1997 Aug;31(4):381-9. doi: 10.1016/s0143-4179(97)90075-5.