PMID- 9308725
OWN - NLM
STAT- MEDLINE
DCOM- 19971021
LR  - 20190722
IS  - 0046-8177 (Print)
IS  - 0046-8177 (Linking)
VI  - 28
IP  - 9
DP  - 1997 Sep
TI  - Immunolocalization of integrins in the normal lung and in pulmonary carcinomas.
PG  - 1018-25
AB  - Cryosections of normal adult lung (n = 7) and pulmonary epithelial tumors, 
      including squamous (n = 8), adeno (n = 8), bronchioloalveolar (n = 5), and large 
      cell (n = 4) carcinomas (SCC, ACC, BAC, LCC), carcinoids (Cd, n = 7), and 
      neuroendocrine carcinomas (NEC) of variable grades (n = 14) were immunostained by 
      the avidin-biotin peroxidase (ABC) method with monoclonal antibodies to the 
      alpha1-6 and alpha(v) and the beta1-4 integrin subunits. Normal adult alveolar 
      septae showed variably intense immunoreactivity for alpha1,3,6 and beta1, whereas 
      reactions for alpha5 and alpha(v) were weaker and uneven; the remaining integrin 
      subunits were not detected. Bronchial and bronchiolar epithelium showed variably 
      intense staining for alpha2.3,6,v and beta1,4. Reactions were often, though not 
      invariably, basally polarized. SCC, ADC, and LCC showed variably intense 
      reactions for alpha2.3,6,v and beta1,4. BAC were strongly and uniformly stained 
      for alpha1.3 and beta1. In Cd, alpha1,2,3,v and beta1 reactions were noted, 
      whereas in NEC, weak alpha1,3 and beta1 staining was detected with only traces of 
      alpha6 and alpha(v). We conclude that alveolar epithelial cells do not express 
      the hemidesmosome-associated, laminin-binding integrin alpha6beta4 of the 
      bronchial epithelium but rather the alpha1beta1 and alpha3beta1, collagen IV, and 
      laminin receptors, respectively. SCC, ADC, and sampled LCC express an integrin 
      repertory qualitatively similar to that of the bronchial epithelium. Distinct 
      from the latter, the integrin repertory of BAC parallels that of the alveolar 
      epithelium by its strong expression of the multipotential alpha1beta1 and 
      alpha3beta1 integrins. NEC tumors do not display the laminin receptors 
      alpha6beta4 and alpha6beta1 shown by SCC and ADC but express instead alpha1beta1, 
      a collagen IV-laminin receptor rarely found in epithelial neoplasms except for 
      BAC. In NEC tumors, integrins, especially alpha2, decrease with 
      dedifferentiation. Notably distinct from epithelial mesotheliomas, the major 
      fibronectin-binding integrin alpha5beta1 was not found in any type of lung 
      carcinoma.
FAU - Koukoulis, G K
AU  - Koukoulis GK
AD  - Department of Pathology, Rush Medical College, Chicago, IL 60612, USA.
FAU - Warren, W H
AU  - Warren WH
FAU - Virtanen, I
AU  - Virtanen I
FAU - Gould, V E
AU  - Gould VE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (Antigens, CD)
RN  - 0 (CD18 Antigens)
RN  - 0 (Integrin alpha1)
RN  - 0 (Integrin alpha2)
RN  - 0 (Integrin alpha3)
RN  - 0 (Integrin alpha5)
RN  - 0 (Integrin alphaV)
RN  - 0 (Integrin beta1)
RN  - 0 (Integrin beta3)
RN  - 0 (Integrin beta4)
RN  - 0 (Integrins)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 143198-26-9 (Integrin alpha4)
SB  - IM
EIN - Hum Pathol 1997 Dec;28(12):1442
MH  - Adenocarcinoma/chemistry
MH  - Adenocarcinoma, Bronchiolo-Alveolar/chemistry
MH  - Antigens, CD/analysis
MH  - CD18 Antigens/analysis
MH  - Carcinoid Tumor/chemistry
MH  - Carcinoma, Large Cell/chemistry
MH  - Carcinoma, Neuroendocrine/chemistry
MH  - Carcinoma, Squamous Cell/chemistry
MH  - Humans
MH  - Immunohistochemistry
MH  - Integrin alpha1
MH  - Integrin alpha2
MH  - Integrin alpha3
MH  - Integrin alpha4
MH  - Integrin alpha5
MH  - Integrin alphaV
MH  - Integrin beta1/analysis
MH  - Integrin beta3
MH  - Integrin beta4
MH  - Integrins/*analysis
MH  - Lung/*chemistry
MH  - Lung Neoplasms/*chemistry
MH  - Platelet Membrane Glycoproteins/analysis
EDAT- 1997/10/06 00:00
MHDA- 1997/10/06 00:01
CRDT- 1997/10/06 00:00
PHST- 1997/10/06 00:00 [pubmed]
PHST- 1997/10/06 00:01 [medline]
PHST- 1997/10/06 00:00 [entrez]
AID - S0046-8177(97)90054-X [pii]
AID - 10.1016/s0046-8177(97)90054-x [doi]
PST - ppublish
SO  - Hum Pathol. 1997 Sep;28(9):1018-25. doi: 10.1016/s0046-8177(97)90054-x.