PMID- 9308913
OWN - NLM
STAT- MEDLINE
DCOM- 19971017
LR  - 20131121
IS  - 1044-1549 (Print)
IS  - 1044-1549 (Linking)
VI  - 17
IP  - 3
DP  - 1997 Sep
TI  - Nitric oxide inhibits inflammatory cytokine production by human alveolar 
      macrophages.
PG  - 279-83
AB  - High levels of nitric oxide (NO) have been reported in exhaled air of asthmatic 
      individuals. Because alveolar macrophages (AM) are major producers of cytokines, 
      and bronchoalveolar lavage fluid (BALF) from asthmatic individuals contains 
      increased levels of inflammatory cytokines, this study was undertaken to 
      determine whether NO modified the production of inflammatory cytokines by human 
      AM. AM were obtained from normal volunteers by fiberoptic bronchoscopy. Tumor 
      necrosis factor-alpha (TNF-alpha) production stimulated by lipopolysaccharide 
      (LPS; 0.5 microg/ml) was measured with an enzyme-linked immunosorbent assay 
      (ELISA). NO generated from 2,2-(hydroxynitrosohydrazono)-bis-ethanamine (DETA 
      NONOate) (0.1 to 1.0 mM) inhibited TNF-alpha secretion in a dose-dependent 
      manner. At 1 mM DETA NONOate, mean inhibition (+/- SEM) of TNF-alpha secretion 
      was 56 +/- 4% (P = 0.002). To determine whether this effect was cytokine 
      specific, interleukin-1beta (IL-1beta) and macrophage inflammatory protein-1alpha 
      (MIP-1alpha) were evaluated, and DETA NONOate was also found to inhibit both of 
      these cytokines. Basal cytokine levels from unstimulated AM were unaffected by 
      NO. These findings indicate that NO is a potent inhibitor of cytokine production 
      by stimulated human AM.
FAU - Thomassen, M J
AU  - Thomassen MJ
AD  - Department of Pulmonary and Critical Care Medicine, The Cleveland Clinic 
      Foundation, Ohio 44195-5038, USA. thomasm@cesmtp.ccf.org
FAU - Buhrow, L T
AU  - Buhrow LT
FAU - Connors, M J
AU  - Connors MJ
FAU - Kaneko, F T
AU  - Kaneko FT
FAU - Erzurum, S C
AU  - Erzurum SC
FAU - Kavuru, M S
AU  - Kavuru MS
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Respir Cell Mol Biol
JT  - American journal of respiratory cell and molecular biology
JID - 8917225
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (Nitroso Compounds)
RN  - 146724-94-9 (2,2'-(hydroxynitrosohydrazono)bis-ethanamine)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Cell Survival/drug effects/immunology
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Cytokines/*biosynthesis/immunology
MH  - Humans
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophage Inflammatory Proteins/antagonists & inhibitors
MH  - Macrophages, Alveolar/cytology/*immunology/*metabolism
MH  - Nitric Oxide/*pharmacology
MH  - Nitroso Compounds/pharmacology
EDAT- 1997/10/06 00:00
MHDA- 1997/10/06 00:01
CRDT- 1997/10/06 00:00
PHST- 1997/10/06 00:00 [pubmed]
PHST- 1997/10/06 00:01 [medline]
PHST- 1997/10/06 00:00 [entrez]
AID - 10.1165/ajrcmb.17.3.2998m [doi]
PST - ppublish
SO  - Am J Respir Cell Mol Biol. 1997 Sep;17(3):279-83. doi: 10.1165/ajrcmb.17.3.2998m.