PMID- 9309112
OWN - NLM
STAT- MEDLINE
DCOM- 19971015
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 98
IP  - 1
DP  - 1997 Oct 1
TI  - Fluorescence in situ hybridization of old G-banded and mounted chromosome 
      preparations.
PG  - 9-15
AB  - An improved method for fluorescence in situ hybridization (FISH) investigation of 
      old, previously G-banded, mounted chromosome preparations with chromosome 
      specific painting probes and centromere-specific probes is described. Before 
      hybridization, the slides are incubated in xylene until the coverslips detach 
      spontaneously; any mechanical manipulation will jeopardize the results. The 
      success of chromosome painting is improved by excluding the regular RNase 
      treatment step prior to hybridization. Additional changes compared with standard 
      FISH protocols are that the 2 x SSC step is omitted, that the amount of added 
      probe is increased approximately 2.5 times, and that the amplification of signals 
      is performed twice. The applicability of the method, which allows double painting 
      with two differently labeled probes using two differently fluorescing colors, was 
      tested on 11 cases involving different chromosome abnormalities and different 
      types of material, including short-term cultures of epithelial and mesenchymal 
      tumors, blood, leukemic bone marrow, and long-term cultures of a cell line 
      derived from an epithelial tumor. Success was achieved even with chromosome 
      preparations that were several years old.
FAU - Gerdes, A M
AU  - Gerdes AM
AD  - Department of Clinical Chemistry, Odense University Hospital, Denmark.
FAU - Pandis, N
AU  - Pandis N
FAU - Bomme, L
AU  - Bomme L
FAU - Dietrich, C U
AU  - Dietrich CU
FAU - Teixeira, M R
AU  - Teixeira MR
FAU - Bardi, G
AU  - Bardi G
FAU - Heim, S
AU  - Heim S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
CIN - Cancer Genet Cytogenet. 1998 Nov;107(1):85-6. PMID: 9809043
MH  - Chromosome Aberrations
MH  - *Chromosome Banding
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Neoplasms/*genetics/pathology
MH  - Tumor Cells, Cultured
EDAT- 1997/10/06 00:00
MHDA- 1997/10/06 00:01
CRDT- 1997/10/06 00:00
PHST- 1997/10/06 00:00 [pubmed]
PHST- 1997/10/06 00:01 [medline]
PHST- 1997/10/06 00:00 [entrez]
AID - S0165460896004074 [pii]
AID - 10.1016/s0165-4608(96)00407-4 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1997 Oct 1;98(1):9-15. doi: 
      10.1016/s0165-4608(96)00407-4.