PMID- 9309115
OWN - NLM
STAT- MEDLINE
DCOM- 19971015
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 98
IP  - 1
DP  - 1997 Oct 1
TI  - p53 loss and point mutations are associated with suppression of apoptosis and 
      progression of CML into myeloid blastic crisis.
PG  - 28-35
AB  - A longitudinal investigation using fluorescence in situ hybridization (FISH) 
      analysis, PCR-SSCP, and in situ detection of apoptosis by the terminal 
      deoxynucleotidyl Transferase (TdT) method was carried out on 13 chronic 
      myelogenous leukemia (CML) patients to study the p53 gene behavior and the 
      apoptotic process during the course of the disease. At diagnosis, FISH showed no 
      loss of the p53 gene on interphase nuclei, and no point mutation was detected by 
      polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) 
      and sequencing. During the disease course, FISH analysis showed a significative 
      loss of allele (LOA) rate for the p53 gene in eight patients that in seven cases 
      was associated with a suppression of apoptotic process and the progressive 
      expansion of the p53+/p53- clone. DNA sequencing showed in two of these eight 
      patients a point mutation on the other allele, consisting in the formation of a 
      stop codon in one case, and in a frameshift mutation in the other. Six patients 
      had a myeloid blastic crisis (BC), five a lymphoid BC, and the other two an 
      erythroid and an undifferentiated BC, respectively. All patients with myeloid BC 
      and the one with undifferentiated BC disclosed a progressive expansion of the 
      clone with p53 loss that was associated with a significant reduction in 
      apoptosis. On the contrary in the 5 patients with lymphoid BC no significant p53 
      LOA rate was observed during the course of the disease. In these patients 
      apoptotic process also persisted in the acute phase although in a lower rate as 
      compared to CP.
FAU - Stuppia, L
AU  - Stuppia L
AD  - Instituto di Biologia e Genetica, Universita di Chieti, Italy.
FAU - Calabrese, G
AU  - Calabrese G
FAU - Peila, R
AU  - Peila R
FAU - Guanciali-Franchi, P
AU  - Guanciali-Franchi P
FAU - Morizio, E
AU  - Morizio E
FAU - Spadano, A
AU  - Spadano A
FAU - Palka, G
AU  - Palka G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Apoptosis/*genetics
MH  - Blast Crisis/*genetics
MH  - DNA Fragmentation
MH  - Female
MH  - *Gene Deletion
MH  - *Genes, p53
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics/pathology/therapy
MH  - Male
MH  - Middle Aged
MH  - *Point Mutation
MH  - Treatment Outcome
EDAT- 1997/10/06 00:00
MHDA- 1997/10/06 00:01
CRDT- 1997/10/06 00:00
PHST- 1997/10/06 00:00 [pubmed]
PHST- 1997/10/06 00:01 [medline]
PHST- 1997/10/06 00:00 [entrez]
AID - S016546089600413X [pii]
AID - 10.1016/s0165-4608(96)00413-x [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1997 Oct 1;98(1):28-35. doi: 
      10.1016/s0165-4608(96)00413-x.