PMID- 9310269
OWN - NLM
STAT- MEDLINE
DCOM- 19971021
LR  - 20190720
IS  - 0304-3835 (Print)
IS  - 0304-3835 (Linking)
VI  - 118
IP  - 1
DP  - 1997 Sep 16
TI  - Ploidy analysis in paraffin-embedded malignant fibrous histiocytoma by DNA 
      cytofluorometry and flourescence in situ hybridization.
PG  - 123-8
AB  - To prove the relationship between chromosomal aberration and DNA ploidy in human 
      malignant fibrous histiocytoma (MFH), fluorescence in situ hybridization (FISH) 
      and DNA cytofluorometry were performed in this study. For FISH study, the nucleus 
      of each tumor cell was isolated from paraffin-embedded tissue of nine MFHs. Five 
      chromosome-specific DNA probes (1p36, 1q12, 8q21.3, 11 centromere, and 17 
      centromere) were hybridized on cell nuclei. Cells with more than three probe 
      signals were regarded as chromosome polysomy. All of the tumors analyzed by FISH 
      had extra copies. The average percentage of polysomy in all tumors was high, 
      ranging from 10.2% to 49.2%. The DNA ploidy patterns, and the percentage of 
      hyperdiploid cells showing a greater DNA content than diploid cells, were 
      obtained from DNA cytofluorometry. Three of nine were diploid patterns and six 
      were non-diploid patterns, and the percentage of hyperdiploid cells in all tumors 
      was high, ranging from 9.1% to 61.9%. The percentage of polysomy could be 
      correlated with the percentage of hyperdiploid cells in each cell. In this study, 
      we found that the DNA ploidy change was closely correlated with aberrations of 
      chromosome copy number in MFH. In addition, the alterations of specific 
      chromosome copy number could be detected in MFH showing diploid cells. Thus, 
      these data indicate that FISH and DNA cytofluorometry are available as a 
      cytogenetic tool for the analysis of interphase nuclei of bone and soft tissue 
      tumors including MFH.
FAU - Murata, H
AU  - Murata H
AD  - Department of Orthopedic Surgery, Kyoto Prefectural University of Medicine, 
      Japan.
FAU - Kusuzaki, K
AU  - Kusuzaki K
FAU - Hirasawa, Y
AU  - Hirasawa Y
FAU - Inazawa, J
AU  - Inazawa J
FAU - Abe, T
AU  - Abe T
FAU - Ashihara, T
AU  - Ashihara T
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Genetic Markers)
RN  - 8002-74-2 (Paraffin)
SB  - IM
MH  - Biopsy
MH  - Centromere
MH  - *Chromosome Aberrations
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 1
MH  - Chromosomes, Human, Pair 11
MH  - Chromosomes, Human, Pair 17
MH  - Chromosomes, Human, Pair 8
MH  - DNA Probes
MH  - DNA, Neoplasm/*analysis
MH  - Flow Cytometry/methods
MH  - Genetic Markers
MH  - Histiocytoma, Benign Fibrous/*genetics/*pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Paraffin
MH  - *Ploidies
EDAT- 1997/10/06 00:00
MHDA- 1997/10/06 00:01
CRDT- 1997/10/06 00:00
PHST- 1997/10/06 00:00 [pubmed]
PHST- 1997/10/06 00:01 [medline]
PHST- 1997/10/06 00:00 [entrez]
AID - S0304-3835(97)00239-5 [pii]
AID - 10.1016/s0304-3835(97)00239-5 [doi]
PST - ppublish
SO  - Cancer Lett. 1997 Sep 16;118(1):123-8. doi: 10.1016/s0304-3835(97)00239-5.