PMID- 9311509
OWN - NLM
STAT- MEDLINE
DCOM- 19971201
LR  - 20190822
IS  - 0903-1936 (Print)
IS  - 0903-1936 (Linking)
VI  - 10
IP  - 9
DP  - 1997 Sep
TI  - Co-stimulation of cultured peripheral blood mononuclear cells from intrinsic 
      asthmatics with exogenous recombinant IL-6 produce high levels of IL-4-dependent 
      IgE.
PG  - 2091-6
AB  - Asthma is an inflammatory airway disorder, traditionally subdivided into 
      extrinsic, immunoglobulin E (IgE)-mediated, and intrinsic asthma of unknown 
      aetiology. IgE synthesis requires contact between T- and B-cells and a signal 
      provided by interleukin (IL)-4, which can be modulated by IL-6. The objective of 
      this study was to evaluate the effects of IL-4 and IL-6 on total IgE synthesis by 
      peripheral blood mononuclear cells from intrinsic and extrinsic asthmatics. 
      Peripheral blood mononuclear cells from intrinsic and extrinsic asthmatic 
      patients and from healthy subjects were cultured and stimulated with pokeweed 
      mitogen, recombinant IL-4 and IL-6. The IgE level in serum and supernatants was 
      measured by an enzyme-linked immunoassay. Serum IgE was significantly lower in 
      intrinsic asthma than in extrinsic asthma, but significantly higher than in 
      control subjects. IgE production by cultured mononuclear cells from extrinsic 
      asthmatics was not modified after exogenous IL-4 and IL-6 addition. However, 
      intrinsic asthmatics showed enhancement of IgE synthesis in response to IL-4 
      stimulation, reaching a threefold increase of the spontaneous IgE values, when 
      simultaneous recombinant IL-4 plus IL-6 stimulus was used. Our results indicate 
      that exogenous recombinant interleukin-6 can significantly upregulate the 
      interleukin-4-dependent immunoglobulin E synthesis in intrinsic asthma. This 
      suggests that immunoglobulin E could also play a role in the pathogenesis of 
      intrinsic asthma, in which an interleukin-6 threshold would be critical.
FAU - Sanchez-Guerrero, I M
AU  - Sanchez-Guerrero IM
AD  - Allergy Unit, Hospital Rafael Mendez, Murcia, Spain.
FAU - Herrero, N
AU  - Herrero N
FAU - Muro, M
AU  - Muro M
FAU - Vegara, R P
AU  - Vegara RP
FAU - Campos, M
AU  - Campos M
FAU - Garcia-Alonso, A M
AU  - Garcia-Alonso AM
FAU - Alvarez, M R
AU  - Alvarez MR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur Respir J
JT  - The European respiratory journal
JID - 8803460
RN  - 0 (Interleukin-6)
RN  - 0 (Pokeweed Mitogens)
RN  - 0 (Recombinant Proteins)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Asthma/*immunology
MH  - Cells, Cultured
MH  - Child
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/*biosynthesis
MH  - Interleukin-4/*pharmacology/physiology
MH  - Interleukin-6/*pharmacology
MH  - Leukocytes, Mononuclear/*immunology
MH  - Male
MH  - Middle Aged
MH  - Pokeweed Mitogens/pharmacology
MH  - Recombinant Proteins/pharmacology
EDAT- 1997/10/06 00:00
MHDA- 1997/10/06 00:01
CRDT- 1997/10/06 00:00
PHST- 1997/10/06 00:00 [pubmed]
PHST- 1997/10/06 00:01 [medline]
PHST- 1997/10/06 00:00 [entrez]
AID - 10.1183/09031936.97.10092091 [doi]
PST - ppublish
SO  - Eur Respir J. 1997 Sep;10(9):2091-6. doi: 10.1183/09031936.97.10092091.