PMID- 9311561 OWN - NLM STAT- MEDLINE DCOM- 19971120 LR - 20191210 IS - 0920-8569 (Print) IS - 0920-8569 (Linking) VI - 14 IP - 3 DP - 1997 TI - Characterization of the polyhedrin gene of Spodoptera litura nuclear polyhedrosis virus. PG - 175-80 AB - The polyhedrin gene (polh) of a characteristically distinct Spodoptera litura nuclear polyhedrosis virus isolate (SIMNPV) is identified in the HindIII-F fragment of the viral DNA. The nucleotide sequence of the 1057 base pair (bp) region of this fragment contains an open reading frame (ORF) without any intervening sequence for coding a polypeptide of 246 amino acids. Analysis of the nucleotide sequence and deduced amino acid sequence indicate that this has more than 70% sequence identity to known polyhedrins. The coding region is preceded by an AT rich region containing the conserved late promoter motif TAAG. The upstream promoter and coding regions of this polh gene are more similar to polh of the NPVs of Spodoptera frugiperda (Sf), Spodoptera exigua (Se) and Panolis flamea (Pf). FAU - Bansal, A AU - Bansal A AD - School of International Health, University of Alabama at Birmingham, 35294-0008, USA. FAU - Bansal, O B AU - Bansal OB FAU - Kumar, M AU - Kumar M FAU - Behera, A K AU - Behera AK FAU - Das, R H AU - Das RH LA - eng PT - Journal Article PL - United States TA - Virus Genes JT - Virus genes JID - 8803967 RN - 0 (DNA, Viral) RN - 0 (Occlusion Body Matrix Proteins) RN - 0 (Viral Proteins) RN - 0 (Viral Structural Proteins) RN - 0 (polyhedrin protein, Nucleopolyhedrovirus) RN - EC 3.1.21.- (Deoxyribonuclease HindIII) SB - IM MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - Blotting, Southern MH - Chromosome Mapping MH - Cloning, Molecular MH - DNA, Viral MH - Deoxyribonuclease HindIII/metabolism MH - Molecular Sequence Data MH - Nucleopolyhedroviruses/*genetics MH - Occlusion Body Matrix Proteins MH - Spodoptera/virology MH - Viral Proteins/*genetics MH - Viral Structural Proteins EDAT- 1997/01/01 00:00 MHDA- 1997/10/06 00:01 CRDT- 1997/01/01 00:00 PHST- 1997/01/01 00:00 [pubmed] PHST- 1997/10/06 00:01 [medline] PHST- 1997/01/01 00:00 [entrez] AID - 10.1023/a:1007975608494 [doi] PST - ppublish SO - Virus Genes. 1997;14(3):175-80. doi: 10.1023/a:1007975608494.