PMID- 9313774
OWN - NLM
STAT- MEDLINE
DCOM- 19971027
LR  - 20190623
IS  - 0006-2952 (Print)
IS  - 0006-2952 (Linking)
VI  - 54
IP  - 4
DP  - 1997 Aug 15
TI  - The effect of pentoxifylline on human neutrophil migration: a possible role for 
      cyclic nucleotides.
PG  - 475-80
AB  - Relatively low concentrations of pentoxifylline caused a stimulation of random 
      migration, while high concentrations inhibited chemotactic migration activated by 
      formyl-methionyl-leucyl-phenylalanine (fMLP). The stimulating effect of 
      pentoxyfylline was partly chemokinetic and partly chemotactic, and was dependent 
      on extracellular calcium. Activation of migration by pentoxifylline was not 
      dependent on the pore size of the micropore filter, indicating that the effect 
      was not mediated by the ability of the drug to induce membrane deformability. 
      Inhibitors of guanylate cyclase and antagonists of cGMP-dependent protein kinase 
      (G-kinase) inhibited stimulation of migration by pentoxifylline. Pentoxyfylline 
      caused a transient increase in cGMP level, while only high concentrations of 
      pentoxifylline caused an increase in cyclic adenosine monophosphate (cAMP) level. 
      It is suggested that the increase of migration is caused by cGMP and is mediated 
      by a G-kinase, while the inhibition of migration at high concentrations of 
      pentoxifylline is mediated by cAMP.
FAU - Elferink, J G
AU  - Elferink JG
AD  - Department of Medical Biochemistry, Leiden University, The Netherlands.
FAU - Huizinga, T W
AU  - Huizinga TW
FAU - de Koster, B M
AU  - de Koster BM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem Pharmacol
JT  - Biochemical pharmacology
JID - 0101032
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - E0399OZS9N (Cyclic AMP)
RN  - H2D2X058MU (Cyclic GMP)
RN  - SD6QCT3TSU (Pentoxifylline)
RN  - SY7Q814VUP (Calcium)
RN  - TBT296U68M (1-Methyl-3-isobutylxanthine)
SB  - IM
MH  - 1-Methyl-3-isobutylxanthine/pharmacology
MH  - Calcium/metabolism
MH  - Cell Movement/drug effects
MH  - Cyclic AMP/*physiology
MH  - Cyclic GMP/*physiology
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*drug effects/physiology
MH  - Pentoxifylline/*pharmacology
EDAT- 1997/08/15 00:00
MHDA- 1997/10/06 00:01
CRDT- 1997/08/15 00:00
PHST- 1997/08/15 00:00 [pubmed]
PHST- 1997/10/06 00:01 [medline]
PHST- 1997/08/15 00:00 [entrez]
AID - S0006-2952(97)00188-3 [pii]
AID - 10.1016/s0006-2952(97)00188-3 [doi]
PST - ppublish
SO  - Biochem Pharmacol. 1997 Aug 15;54(4):475-80. doi: 10.1016/s0006-2952(97)00188-3.