PMID- 9315335
OWN - NLM
STAT- MEDLINE
DCOM- 19971230
LR  - 20190905
IS  - 8756-3282 (Print)
IS  - 1873-2763 (Linking)
VI  - 21
IP  - 4
DP  - 1997 Oct
TI  - Monocyte chemoattractant protein-1 induces monocyte recruitment that is 
      associated with an increase in numbers of osteoblasts.
PG  - 321-7
AB  - Monocyte chemoattractant protein-1 (MCP-1) is a member of the chemokine family of 
      cytokines. The principal function of MCP-1 is thought to be the stimulation of 
      monocyte recruitment. Monocyte products are potential regulators of bone cell 
      activity. Growth factors produced by monocytes may stimulate bone formation, 
      while cytokines such as IL-1 and IL-6 can induce bone resorption. To determine 
      whether MCP-1 enhances recruitment of monocytes during bone healing, studies were 
      carried out in which MCP-1 was applied to osseous sites in vivo. Changes in 
      monocyte number were determined by immunohistochemistry using the antibody ED-1 
      specific for peripheral monocytic cells. The effect of MCP-1 on osteoblast number 
      was determined by counting the number of alkaline phosphatase positive cells in 
      close proximity to bone. For comparison, osteoblast number was also determined 
      following stimulation with platelet-derived growth factor (PDGF)-BB plus IGF-1 in 
      vivo. Results indicate that MCP-1 stimulated a large increase in monocyte 
      recruitment compared to vehicle alone. An increase in monocytes induced by MCP-1 
      was associated with an increase in the number of osteoblasts lining the bone 
      surface, although not to the same magnitude as a positive control, PDGF-BB, and 
      IGF-1. These results indicate that MCP-1 induces the recruitment of monocytes to 
      bone and suggest that the recruitment is associated with an increase in 
      osteoblast number. This is likely to occur via indirect mechanisms, because MCP-1 
      did not directly enhance DNA synthesis in osteoblastic cells in vitro. Thus, 
      activated mononuclear phagocytes may play an important role in osseous wound 
      healing by stimulating proliferation of osteoblastic cells, presumably through 
      the elaboration of growth factors.
FAU - Posner, L J
AU  - Posner LJ
AD  - Division of Oral Biology, Boston University School of Dental Medicine, MA, USA.
FAU - Miligkos, T
AU  - Miligkos T
FAU - Gilles, J A
AU  - Gilles JA
FAU - Carnes, D L
AU  - Carnes DL
FAU - Taddeo, D R
AU  - Taddeo DR
FAU - Graves, D T
AU  - Graves DT
LA  - eng
GR  - DE07559/DE/NIDCR NIH HHS/United States
GR  - DE11254/DE/NIDCR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Bone
JT  - Bone
JID - 8504048
RN  - 0 (Anticonvulsants)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (Proto-Oncogene Proteins c-sis)
RN  - 1B56C968OA (Becaplermin)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 9013-72-3 (Hemocyanins)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase
MH  - Animals
MH  - Anticonvulsants/pharmacology
MH  - Becaplermin
MH  - Cell Count/drug effects
MH  - Chemokine CCL2/*pharmacology
MH  - Hemocyanins
MH  - Immunohistochemistry
MH  - Insulin-Like Growth Factor I/pharmacology
MH  - Male
MH  - Monocytes/*drug effects/pathology
MH  - Osteitis/chemically induced/pathology
MH  - Osteoblasts/*drug effects/pathology
MH  - Platelet-Derived Growth Factor/pharmacology
MH  - Proto-Oncogene Proteins c-sis
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1997/10/07 00:00
MHDA- 1997/10/07 00:01
CRDT- 1997/10/07 00:00
PHST- 1997/10/07 00:00 [pubmed]
PHST- 1997/10/07 00:01 [medline]
PHST- 1997/10/07 00:00 [entrez]
AID - S8756328297001543 [pii]
AID - 10.1016/s8756-3282(97)00154-3 [doi]
PST - ppublish
SO  - Bone. 1997 Oct;21(4):321-7. doi: 10.1016/s8756-3282(97)00154-3.