PMID- 9315384
OWN - NLM
STAT- MEDLINE
DCOM- 19971119
LR  - 20190826
IS  - 0305-1870 (Print)
IS  - 0305-1870 (Linking)
VI  - 24
IP  - 9-10
DP  - 1997 Sep-Oct
TI  - Detection of weakly expressed genes in the rostral ventrolateral medulla of the 
      rat using micropunch and reverse transcription-polymerase chain reaction 
      techniques.
PG  - 755-9
AB  - 1. The present study describes the use of reverse transcription-polymerase chain 
      reaction (RT-PCR) to detect weakly expressed neurotransmitter receptor mRNA in 
      tissue micropunched from the rostral ventrolateral medulla (RVLM) and other 
      discrete areas of the medulla oblongata of the rat. 2. Micropunches were made 
      from 240 microns transverse medullary sections. Punched regions included the 
      RVLM, hypoglossal nucleus (XIIn), ventrolateral subnucleus of the nucleus tractus 
      solitarius (NTS) and spinal trigeminal nucleus (STN). RNA was extracted and 
      reverse transcribed into cDNA, which was probed for the presence of seven genes: 
      glyceraldehyde phosphate dehydrogenase (GAPDH), neuron-specific enolase (NSE), 
      tyrosine hydroxylase (TH), phenylethanolamine N-methyltransferase (PNMT), 
      glucocorticoid receptor (GCR), mineralocorticoid receptor (MCR) and the adenosine 
      5'-triphosphate (ATP) receptor subunit P2X2-1. Each transcript was detected using 
      a semi-nested PCR protocol, which used three primers. 3. Tyrosine hydroxylase was 
      detected in the RVLM and NTS and PNMT was also detected in the RVLM, which agrees 
      with the distribution of catecholamine neurons in the medulla. Expression of GCR 
      mRNA was detected in the RVLM and the XIIn but not in the NTS (it was not probed 
      for in the STN punches). The P2X2-1 receptor message was detected in all areas. 
      Expression of MCR mRNA was detected in the RVLM only. 4. This method offers a 
      simple way to detect the presence of low-abundance receptor mRNA in discrete 
      brain regions.
FAU - Comer, A M
AU  - Comer AM
AD  - Department of Physiology, University of Auckland, New Zealand.
FAU - Yip, S
AU  - Yip S
FAU - Lipski, J
AU  - Lipski J
LA  - eng
SI  - GENBANK/L22651
SI  - GENBANK/L43511
SI  - GENBANK/M11931
SI  - GENBANK/M14053
SI  - GENBANK/M36074
SI  - GENBANK/U14414
SI  - GENBANK/X02231
SI  - GENBANK/X14211
PT  - Journal Article
PL  - Australia
TA  - Clin Exp Pharmacol Physiol
JT  - Clinical and experimental pharmacology & physiology
JID - 0425076
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Neurotransmitter)
SB  - IM
MH  - Animals
MH  - Gene Expression Regulation/*physiology
MH  - Male
MH  - Medulla Oblongata/cytology/*physiology
MH  - Molecular Sequence Data
MH  - Needles
MH  - Polymerase Chain Reaction/*methods
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Neurotransmitter/biosynthesis/genetics
MH  - Specimen Handling
EDAT- 1997/10/07 00:00
MHDA- 1997/10/07 00:01
CRDT- 1997/10/07 00:00
PHST- 1997/10/07 00:00 [pubmed]
PHST- 1997/10/07 00:01 [medline]
PHST- 1997/10/07 00:00 [entrez]
AID - 10.1111/j.1440-1681.1997.tb02127.x [doi]
PST - ppublish
SO  - Clin Exp Pharmacol Physiol. 1997 Sep-Oct;24(9-10):755-9. doi: 
      10.1111/j.1440-1681.1997.tb02127.x.