PMID- 9316923
OWN - NLM
STAT- MEDLINE
DCOM- 19971120
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 35
IP  - 10
DP  - 1997 Oct
TI  - Evaluation of a fluorescence-labelled oligonucleotide probe targeting 23S rRNA 
      for in situ detection of Salmonella serovars in paraffin-embedded tissue sections 
      and their rapid identification in bacterial smears.
PG  - 2642-8
AB  - A method for the detection of Salmonella based on fluorescence in situ 
      hybridization (FISH) has been developed and applied for the direct detection of 
      Salmonella in pure cultures and in formalin-fixed, paraffin-embedded tissue 
      sections. On the basis of the 23S rRNA gene sequences representing all of the S. 
      enterica subspecies and S. bongori, an 18-mer oligonucleotide probe was selected. 
      The specificity of the probe was tested by in situ hybridization to bacterial 
      cell smears of pure cultures. Forty-nine of 55 tested Salmonella serovars 
      belonging to subspecies I, II, IIIb, IV, and VI hybridized with the probe. The 
      probe did not hybridize to serovars from subspecies IIIa (S. arizonae) or to S. 
      bongori. No cross-reaction to 64 other strains of the family Enterobacteriaceae 
      or 18 other bacterial strains outside this family was observed. The probe was 
      tested with sections of formalin-fixed, paraffin-embedded tissue from 
      experimentally infected mice or from animals with a history of clinical 
      salmonellosis. In these tissue sections the probe hybridized specifically to 
      Salmonella serovars, allowing for the detection of single bacterial cells. The 
      development of a fluorescence-labelled specific oligonucleotide probe makes the 
      FISH technique a promising tool for the rapid identification of S. enterica in 
      bacterial smears, as well as for the detection of S. enterica in histological 
      tissue sections.
FAU - Nordentoft, S
AU  - Nordentoft S
AD  - Danish Veterinary Laboratory, Copenhagen V. sn@svs.dk
FAU - Christensen, H
AU  - Christensen H
FAU - Wegener, H C
AU  - Wegener HC
LA  - eng
SI  - GENBANK/U88706
SI  - GENBANK/U88707
SI  - GENBANK/U88708
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Ribosomal, 23S)
RN  - 8002-74-2 (Paraffin)
SB  - IM
MH  - Animals
MH  - *Bacterial Typing Techniques
MH  - Cattle
MH  - Enterobacteriaceae/genetics
MH  - Enterocolitis/microbiology/veterinary
MH  - Evaluation Studies as Topic
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Mice
MH  - Microtomy
MH  - Molecular Sequence Data
MH  - Nucleic Acid Hybridization
MH  - *Oligonucleotide Probes
MH  - Paraffin
MH  - Pneumonia, Bacterial/microbiology/veterinary
MH  - RNA, Ribosomal, 23S/*genetics
MH  - Salmonella/*classification/genetics/isolation & purification
MH  - Salmonella enterica/classification/genetics/isolation & purification
MH  - Sensitivity and Specificity
MH  - Species Specificity
MH  - Swine
MH  - Tissue Embedding
PMC - PMC230026
EDAT- 1997/10/08 00:00
MHDA- 1997/10/08 00:01
PMCR- 1997/10/01
CRDT- 1997/10/08 00:00
PHST- 1997/10/08 00:00 [pubmed]
PHST- 1997/10/08 00:01 [medline]
PHST- 1997/10/08 00:00 [entrez]
PHST- 1997/10/01 00:00 [pmc-release]
AID - 10.1128/jcm.35.10.2642-2648.1997 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1997 Oct;35(10):2642-8. doi: 10.1128/jcm.35.10.2642-2648.1997.