PMID- 9321425
OWN - NLM
STAT- MEDLINE
DCOM- 19971202
LR  - 20191210
IS  - 0093-7711 (Print)
IS  - 0093-7711 (Linking)
VI  - 46
IP  - 6
DP  - 1997
TI  - Cloning, structural analysis, and expression of the pig IgE epsilon chain.
PG  - 461-8
AB  - As a step in the evolutionary studies of immunoglobulin E (IgE) and for the 
      purpose of developing new reagents that will facilitate a more detailed analysis 
      of IgE-mediated inflammatory reactions in a large animal model, we here present 
      the cloning of the epsilon chain of IgE in the domestic pig (Sus scrufa). A 
      partial cDNA clone for the epsilon chain of pig IgE was isolated by polymerase 
      chain reaction (PCR) amplification using degenerate primers directed against 
      conserved regions in the second (CH2) and the fourth (CH4) constant domains of 
      IgE. cDNA derived from mRNA isolated from the spleen and lymph nodes of a pig 
      actively sensitized with a protein extract from the nematode Ascaris suum was 
      used as template. Screening of a spleen cDNA library with the partial cDNA clone 
      as probe resulted in isolation of a clone that contained the entire coding 
      region. The nucleotide sequence was determined and was found to conform with the 
      previously identified mammalian epsilon-chain sequences. The highest degree of 
      similarity was found to sheep IgE. A DNA construct encoding a baculovirus signal 
      sequence, a histidine hexapeptide, and the CH2-CH3-CH4 domains of the pig IgE 
      epsilon chain was obtained by PCR amplification. The construct was ligated into 
      the baculovirus expression vector pVL1392. Infection of High Five insect cells 
      with recombinant baculovirus resulted in expression and secretion of a soluble 6 
      x His-CH2-CH3-CH4 protein product.
FAU - Vernersson, M
AU  - Vernersson M
AD  - Department of Medical Immunology and Microbiology, University of Uppsala, Sweden.
FAU - Pejler, G
AU  - Pejler G
FAU - Kristersson, T
AU  - Kristersson T
FAU - Alving, K
AU  - Alving K
FAU - Hellman, L
AU  - Hellman L
LA  - eng
SI  - GENBANK/U96100
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (DNA, Complementary)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Immunoglobulin E/chemistry/*genetics
MH  - Molecular Sequence Data
MH  - Nucleopolyhedroviruses/genetics
MH  - Protein Conformation
MH  - Sequence Homology, Amino Acid
MH  - Spodoptera
MH  - Swine
EDAT- 1997/01/01 00:00
MHDA- 1997/10/08 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/10/08 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1007/s002510050306 [doi]
PST - ppublish
SO  - Immunogenetics. 1997;46(6):461-8. doi: 10.1007/s002510050306.