PMID- 932212
OWN - NLM
STAT- MEDLINE
DCOM- 19760823
LR  - 20181113
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 58
IP  - 1
DP  - 1976 Jul
TI  - Intestinal myoelectric activity in response to live Vibrio cholerae and cholera 
      enterotoxin.
PG  - 91-6
AB  - The myoelectric response of the rabbit ileum was studied in response to live 
      Vibrio cholerae culture, a whole cell lysate of cholera, and the purified 
      enterotoxin. Each cholera preparation produced a series of highly organized 
      migrating action potential complexes (MAPC). An MAPC was defined as action 
      potential discharge with a duration of 2.5 s or longer, followed by similar 
      activity on at least one other consecutive electrode site. The mean and modal 
      onset time of MAPC activity occurred 4 h after the infection with live Vibrio 
      cholerae culture, the freeze-dried whole cell lysate preparation, or the purified 
      enterotoxin. After the onset of activity this pattern persisted for the duration 
      of the recording period (up to 12 h). The MAPC had a mean propagation velocity of 
      0.85+/-0.07 cm/s (mean+/-SEM), which remained constant with time. Direct visual 
      observation of the loop revealed that the MAPC's resulted in contractions that 
      propelled intraluminal contents in an aborad direction. The mean fluid output 
      from the 12-cm ileal loops was 6.4+/-1.1 ml/h (mean+/-SEM). Control experiments 
      consisted of recordings from: (a) a ligated ileal loop into which nothing was 
      placed; (b) a ligated ileal loop into which either uninfected culture broth or 
      0.9% NaCl solution was injected; (c) a ligated ileal loop infused with 0.9% NaCl 
      solution at a rate of 11.2 ml/h, and (d) rapid injection of 1.0, 2.5, 5.0, or 
      10.0-ml boluses of 0.9% NaCl into the proximal catheter. MAPC activity was not 
      observed in any of the control experiments. These studies indicate that in 
      addition to a secretory component to cholera, there exists a highly organized 
      MAPC that results in contractions that propel intraluminal contents in an aborad 
      direction.
FAU - Mathias, J R
AU  - Mathias JR
FAU - Carlson, G M
AU  - Carlson GM
FAU - DiMarino, A J
AU  - DiMarino AJ
FAU - Bertiger, G
AU  - Bertiger G
FAU - Morton, H E
AU  - Morton HE
FAU - Cohen, S
AU  - Cohen S
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Enterotoxins)
SB  - IM
MH  - Action Potentials
MH  - Animals
MH  - Cholera/*microbiology
MH  - Electrophysiology
MH  - Enterotoxins/isolation & purification/*pharmacology
MH  - Female
MH  - Freeze Drying
MH  - Ileum/*physiology
MH  - Male
MH  - Muscle, Smooth/*physiology
MH  - Rabbits
MH  - *Vibrio cholerae
PMC - PMC333159
EDAT- 1976/07/01 00:00
MHDA- 1976/07/01 00:01
PMCR- 1976/07/01
CRDT- 1976/07/01 00:00
PHST- 1976/07/01 00:00 [pubmed]
PHST- 1976/07/01 00:01 [medline]
PHST- 1976/07/01 00:00 [entrez]
PHST- 1976/07/01 00:00 [pmc-release]
AID - 10.1172/JCI108464 [doi]
PST - ppublish
SO  - J Clin Invest. 1976 Jul;58(1):91-6. doi: 10.1172/JCI108464.