PMID- 9322967
OWN - NLM
STAT- MEDLINE
DCOM- 19971023
LR  - 20081121
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 138
IP  - 10
DP  - 1997 Oct
TI  - Human neutrophils express GH-N gene transcripts and the pituitary transcription 
      factor Pit-1b.
PG  - 4481-4
AB  - Since GH stimulates the development and function of granulocytes, we investigated 
      the expression of GH in granulocyte subsets. By immunocytochemistry, 25 +/- 7% of 
      the human neutrophils were shown to express immunoreactive GH, whereas 
      eosinophils were negative. Reversed transcription (RT)-PCR analysis demonstrated 
      GH mRNA in neutrophils. Restriction analysis revealed that neutrophils express 
      the GH-N gene but not the GH-V gene. Furthermore, we demonstrated by western blot 
      analysis that neutrophils express an alternatively spliced variant of the 
      pituitary transcription factor Pit-1, designated Pit-1b.
FAU - Kooijman, R
AU  - Kooijman R
AD  - Department of Pharmacology, Medical School, Free University of Brussels (V.U.B.), 
      Belgium.
FAU - Berus, D
AU  - Berus D
FAU - Malur, A
AU  - Malur A
FAU - Delhase, M
AU  - Delhase M
FAU - Hooghe-Peters, E L
AU  - Hooghe-Peters EL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (POU1F1 protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factor Pit-1)
RN  - 0 (Transcription Factors)
RN  - 9002-72-6 (Growth Hormone)
SB  - IM
MH  - Adenoma/chemistry/pathology
MH  - Adult
MH  - Blotting, Western
MH  - DNA-Binding Proteins/*biosynthesis/*genetics/physiology
MH  - Gene Expression Regulation
MH  - Genetic Variation
MH  - Granulocytes/chemistry/cytology/metabolism
MH  - Growth Hormone/*biosynthesis/*genetics/physiology
MH  - Humans
MH  - Immunohistochemistry
MH  - Middle Aged
MH  - Neutrophils/chemistry/cytology/*metabolism
MH  - Pituitary Gland/*chemistry/cytology
MH  - Pituitary Neoplasms/chemistry/pathology
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/analysis/genetics/metabolism
MH  - Transcription Factor Pit-1
MH  - Transcription Factors/*biosynthesis/*genetics/physiology
MH  - *Transcription, Genetic
EDAT- 1997/10/10 00:00
MHDA- 1997/10/10 00:01
CRDT- 1997/10/10 00:00
PHST- 1997/10/10 00:00 [pubmed]
PHST- 1997/10/10 00:01 [medline]
PHST- 1997/10/10 00:00 [entrez]
AID - 10.1210/endo.138.10.5568 [doi]
PST - ppublish
SO  - Endocrinology. 1997 Oct;138(10):4481-4. doi: 10.1210/endo.138.10.5568.