PMID- 9322976
OWN - NLM
STAT- MEDLINE
DCOM- 19971023
LR  - 20131121
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 138
IP  - 10
DP  - 1997 Oct
TI  - Hormonal regulation of monocyte chemoattractant protein-1 messenger ribonucleic 
      acid expression in corpora lutea.
PG  - 4517-20
AB  - Monocyte chemoattractant protein-1 (MCP-1) is a potent chemokine that attracts 
      monocytes and macrophages. It is known that macrophages accumulate in the corpus 
      luteum (CL) during luteal regression in many species. In this study, we 
      investigated the regulation of MCP-1 mRNA in ovine and bovine CL during 
      prostaglandin (PG) F2alpha-induced luteolysis, after LH treatment, or after 
      pharmacologic activation of the protein kinase (PK) A or PKC intracellular 
      effector systems. In experiment 1, ewes on day 11 or 12 of the estrous cycle were 
      infused with saline or PGF2alpha. PGF2alpha increased MCP-1 mRNA at 1 and 4 h 
      after treatment. MCP-1 mRNA returned to basal level at 12 h and increased again 
      at 24 h post treatment. In experiment 2, ewes received saline, PGF2alpha, phorbol 
      12-myristate 13-acetate (PMA), luteinizing hormone (LH), or forskolin infusion 
      and CL were collected at 0 (untreated), 4, 12, or 24 h after infusion. Similar to 
      experiment 1, PGF2alpha induced MCP-1 mRNA at 4 and 24 h post treatment. PMA 
      increased mRNA for MCP-1 at 4, 12, and 24 h. Treatment with LH or forskolin 
      transiently decreased MCP-1 mRNA expression. In experiment 3, cows were treated 
      with a luteolytic dose (25 mg) of PGF2alpha on day 4 or day 11 of estrous cycle 
      and expression of MCP-1 mRNA was quantified. Steady-state concentrations of mRNA 
      for MCP-1 were induced by PGF2alpha treatment only in mid-cycle CL but not in 
      early CL. In summary, administration of PGF2alpha or activation of PKC induced 
      MCP-1 mRNA expression. Expression of MCP-1 may be important for stimulating 
      immune processes during luteal regression.
FAU - Tsai, S J
AU  - Tsai SJ
AD  - Endocrinology-Reproductive Physiology Program and Dairy Science Department, 
      University of Wisconsin-Madison, 53706, USA.
FAU - Juengel, J L
AU  - Juengel JL
FAU - Wiltbank, M C
AU  - Wiltbank MC
LA  - eng
GR  - HD-11590/HD/NICHD NIH HHS/United States
GR  - HD-32623/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Chemokine CCL2)
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Messenger)
RN  - 1F7A44V6OU (Colforsin)
RN  - 9002-62-4 (Prolactin)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - 9007-49-2 (DNA)
RN  - B7IN85G1HY (Dinoprost)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cattle
MH  - Chemokine CCL2/analysis/*genetics/metabolism
MH  - Colforsin/pharmacology
MH  - Corpus Luteum/chemistry/*metabolism
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism/physiology
MH  - DNA/analysis/chemistry/genetics
MH  - DNA Primers/analysis/chemistry/genetics
MH  - Dinoprost/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Activation/drug effects
MH  - Female
MH  - Linear Models
MH  - Luteinizing Hormone/*pharmacology
MH  - Luteolysis/physiology
MH  - Ovulation/physiology
MH  - Polymerase Chain Reaction
MH  - Prolactin/*pharmacology
MH  - Protein Kinase C/metabolism/physiology
MH  - RNA, Messenger/*analysis/chemistry/genetics
MH  - Sheep
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Time Factors
EDAT- 1997/10/10 00:00
MHDA- 1997/10/10 00:01
CRDT- 1997/10/10 00:00
PHST- 1997/10/10 00:00 [pubmed]
PHST- 1997/10/10 00:01 [medline]
PHST- 1997/10/10 00:00 [entrez]
AID - 10.1210/endo.138.10.5577 [doi]
PST - ppublish
SO  - Endocrinology. 1997 Oct;138(10):4517-20. doi: 10.1210/endo.138.10.5577.