PMID- 9323541
OWN - NLM
STAT- MEDLINE
DCOM- 19971120
LR  - 20190831
IS  - 1387-2273 (Print)
IS  - 1387-2273 (Linking)
VI  - 696
IP  - 2
DP  - 1997 Aug 29
TI  - Quantitative determination of several synthetic corticosteroids by gas 
      chromatography-mass spectrometry after purification by immunoaffinity 
      chromatography.
PG  - 203-15
AB  - A study was conducted to test a multiresidue analytical procedure for detecting 
      and quantifying several corticosteroids on which the European Union imposes 
      maximum residue limits (MRLs). Primary extracts from different matrices (liver, 
      milk, urine, faeces) were first purified on C18 cartridges. A new immunoaffinity 
      clean-up step was included. The immunoaffinity gel was used to purify several 
      corticosteroids simultaneously with enrichment of the corresponding fractions. 
      The extracts were treated with an aqueous solution of pyridinium chlorochromate 
      to fully oxidise all corticosteroids and to facilitate their extraction with 
      dichloromethane. After evaporation, the final extract was reconstituted with 
      toluene before injection into the GC-MS apparatus. The analysis was performed in 
      the CI-negative ionisation mode using ammonia as the reactant gas. The estimated 
      detection and quantification limits were, respectively, 0.25 and 0.5 ppb or 
      lower. Overall, the method is reproducible to within 20%. Recovery is between 50 
      and 80% according to the corticosteroid.
FAU - Delahaut, P
AU  - Delahaut P
AD  - CER, Laboratoire d'Hormonologie, Marloie, Belgium.
FAU - Jacquemin, P
AU  - Jacquemin P
FAU - Colemonts, Y
AU  - Colemonts Y
FAU - Dubois, M
AU  - Dubois M
FAU - De Graeve, J
AU  - De Graeve J
FAU - Deluyker, H
AU  - Deluyker H
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Chromatogr B Biomed Sci Appl
JT  - Journal of chromatography. B, Biomedical sciences and applications
JID - 9714109
RN  - 0 (Adrenal Cortex Hormones)
SB  - IM
MH  - Adrenal Cortex Hormones/*analysis/isolation & purification
MH  - Animals
MH  - Cattle
MH  - Chromatography, Affinity/*methods
MH  - Feces/chemistry
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Liver/chemistry
MH  - Mass Spectrometry
MH  - Milk/chemistry
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Urine/chemistry
EDAT- 1997/08/29 00:00
MHDA- 1997/11/05 00:01
CRDT- 1997/08/29 00:00
PHST- 1997/08/29 00:00 [pubmed]
PHST- 1997/11/05 00:01 [medline]
PHST- 1997/08/29 00:00 [entrez]
AID - 10.1016/s0378-4347(97)00222-3 [doi]
PST - ppublish
SO  - J Chromatogr B Biomed Sci Appl. 1997 Aug 29;696(2):203-15. doi: 
      10.1016/s0378-4347(97)00222-3.