PMID- 9323597
OWN - NLM
STAT- MEDLINE
DCOM- 19971215
LR  - 20210217
IS  - 0022-2275 (Print)
IS  - 0022-2275 (Linking)
VI  - 38
IP  - 9
DP  - 1997 Sep
TI  - Degranulation of cutaneous mast cells induces transendothelial transport and 
      local accumulation of plasma LDL in rat skin in vivo.
PG  - 1877-87
AB  - In severe hypercholesterolemia and sometimes even in normolipemia, 
      lipoprotein-derived cholesterol accumulates in the skin, forming xanthomas, but 
      the local factors triggering this accumulation are not known. We therefore 
      studied the effect of immunoglobulin E (IgE)-mediated stimulation of cutaneous 
      mast cells on the transport of low density lipoprotein (LDL) from plasma to skin 
      during the passive cutaneous anaphylaxis (PCA) reaction in rats. PCA sites were 
      produced in rats by giving intracutaneous injections of rat serum containing high 
      levels of IgE antibodies against ovalbumin. When ovalbumin was then injected 
      intravenously into the rats, the skin histamine content at the sensitized skin 
      sites decreased, reflecting degranulation of the passively sensitized 
      (IgE-containing) mast cells at these sites, with ensuing release of histamine 
      from the mast cells. Concomitant intravenous injection of increasing amounts of 
      human [14C]sucrose-LDL led to rapid and dose-dependent increases in the skin 
      [14C]sucrose-LDL content. These (maximally about 40-fold) increases were strictly 
      localized to the PCA reaction sites: no such increase in [14C]sucrose-LDL content 
      was observed in nonsensitized skin areas or in any other tissue of the rats. The 
      increase in skin [14C]sucrose-LDL content could be blocked (by about 80%) with a 
      combination of H1 and H2 receptor antagonists, and could be mimicked by 
      intradermal injection of histamine. This study demonstrates an increase in plasma 
      LDL influx into the skin at sites where mast cells are stimulated, and so 
      suggests that mast cells play a role in the accumulation of LDL cholesterol that 
      occurs in the skin when xanthomas form.
FAU - Ma, H
AU  - Ma H
AD  - Wihuri Research Institute, Helsinki, Finland.
FAU - Kovanen, P T
AU  - Kovanen PT
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Lipid Res
JT  - Journal of lipid research
JID - 0376606
RN  - 0 (LDL-sucrose)
RN  - 0 (Lipoproteins, LDL)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 57-50-1 (Sucrose)
SB  - IM
MH  - Animals
MH  - Biological Transport, Active
MH  - Cell Degranulation
MH  - Endothelium, Vascular/metabolism
MH  - Histamine Release
MH  - Humans
MH  - Immunoglobulin E/metabolism
MH  - Lipoproteins, LDL/blood/*metabolism
MH  - Male
MH  - Mast Cells/immunology/*physiology
MH  - Passive Cutaneous Anaphylaxis
MH  - Rats
MH  - Rats, Wistar
MH  - Skin/cytology/*immunology/*metabolism
MH  - Sucrose/blood/metabolism
MH  - Xanthomatosis/etiology/metabolism
EDAT- 1997/11/05 00:00
MHDA- 1997/11/05 00:01
CRDT- 1997/11/05 00:00
PHST- 1997/11/05 00:00 [pubmed]
PHST- 1997/11/05 00:01 [medline]
PHST- 1997/11/05 00:00 [entrez]
AID - S0022-2275(20)37162-5 [pii]
PST - ppublish
SO  - J Lipid Res. 1997 Sep;38(9):1877-87.