PMID- 9324055
OWN - NLM
STAT- MEDLINE
DCOM- 19971103
LR  - 20190813
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 132
IP  - 1-2
DP  - 1997 Sep 19
TI  - Stage-specific expression and phosphorylation of retinoblastoma protein (pRb) in 
      the rat seminiferous epithelium.
PG  - 137-48
AB  - To assess the potential role of retinoblastoma protein (pRb) in the regulation of 
      cell cycle during spermatogenesis, the expression of retinoblastoma (Rb) mRNA and 
      protein, as well as the phosphorylation states of pRb, in the rat seminiferous 
      epithelial cycle, were studied. Two transcripts, 5.4 kb and 3.4 kb long, were 
      detected in total RNA from the adult rat testis and only the 5.4 kb transcript 
      was detected in poly (A)+-RNA from 8, 14 and 23-day old rat testes by Northern 
      hybridization. Polysome analysis revealed that only a small portion of both Rb 
      transcripts could be efficiently translated. By in situ hybridization, Rb mRNA 
      was localized to germ cells from stage V pachytene spermatocytes to step 13 
      spermatids along the epithelial cycle. pRb immunoreactivity was detected in 
      Sertoli cells and spermatogonia at all stages, as well as in the elongated steps 
      14-19 spermatids by immunohistochemistry. The amount of pRb and the 
      phosphorylation status varied in a stage-specific manner in Western blots. These 
      results show that pRb is expressed in the rat seminiferous epithelium in a cyclic 
      fashion and suggest that it is involved in the regulation of proliferation of 
      spermatogonia and maintenance of the differentiation status of Sertoli cells and 
      spermatids.
FAU - Yan, W
AU  - Yan W
AD  - Department of Medical Genetics and Center for Reproductive and Developmental 
      Medicine, University of Turku, Finland. weiyan@utu.fi
FAU - West, A
AU  - West A
FAU - Toppari, J
AU  - Toppari J
FAU - Lahdetie, J
AU  - Lahdetie J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (RNA, Messenger)
RN  - 0 (Retinoblastoma Protein)
SB  - IM
MH  - Animals
MH  - Gene Expression Regulation
MH  - In Situ Hybridization
MH  - Male
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Retinoblastoma Protein/genetics/*metabolism
MH  - Seminiferous Epithelium/cytology/*metabolism
MH  - Spermatogenesis
EDAT- 1997/11/05 00:00
MHDA- 1997/11/05 00:01
CRDT- 1997/11/05 00:00
PHST- 1997/11/05 00:00 [pubmed]
PHST- 1997/11/05 00:01 [medline]
PHST- 1997/11/05 00:00 [entrez]
AID - S0303-7207(97)00129-9 [pii]
AID - 10.1016/s0303-7207(97)00129-9 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 1997 Sep 19;132(1-2):137-48. doi: 
      10.1016/s0303-7207(97)00129-9.