PMID- 9324066
OWN - NLM
STAT- MEDLINE
DCOM- 19971024
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 61
IP  - 13
DP  - 1997
TI  - Cocaine-induced increase of Mn-SOD in adult rat liver cells.
PG  - 1245-51
AB  - Cultured adult rat liver cells (non-induced) were exposed to two different 
      concentrations (2 microg/ml and 4 microg/ml) of cocaine-HCI for 1-3 hours. 
      Cocaine-treated rat liver cells showed a significant increase in manganese 
      superoxide dismutase (Mn-SOD) activity with time. Coinciding the rise in Mn-SOD 
      activity was the Mn-SOD mRNA transcript, which also demonstrated a time-dependent 
      elevation in its level (p<0.005). At the end of 1 and 3 hr. of cocaine exposure, 
      the increase in Mn-SOD activity was 23% (p<0.05) and 39% (p<0.005), respectively. 
      In contrast, activities of catalase (CAT) and glutathione peroxidase (GSH-px) 
      were significantly diminished. Induction of Mn-SOD mRNA by cocaine (4 microg/ml) 
      was inhibited by actinomycin D (4 microM), revealing that the up regulation of 
      Mn-SOD involved transcriptional activation. The increase in Mn-SOD was 
      accompanied by a decrease in liver cell integrity, indicated by increased lactate 
      dehydrogenase (LDH) release by rat liver cells. These results suggest that 
      antioxidant enzymes may play a critical role in cocaine-induced liver injury.
FAU - Devi, B G
AU  - Devi BG
AD  - Research Institute on Addictions, Buffalo, New York 14203, USA. devi@ria.org
FAU - Chan, A W
AU  - Chan AW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Nucleic Acid Synthesis Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - I5Y540LHVR (Cocaine)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Catalase/metabolism
MH  - Cocaine/*pharmacology
MH  - Dactinomycin/pharmacology
MH  - Gene Expression Regulation, Enzymologic/drug effects
MH  - Glutathione Peroxidase/metabolism
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Liver/*enzymology
MH  - Male
MH  - Nucleic Acid Synthesis Inhibitors/pharmacology
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Superoxide Dismutase/*biosynthesis/genetics
EDAT- 1997/01/01 00:00
MHDA- 1997/11/04 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/11/04 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - S0024320597006693 [pii]
AID - 10.1016/s0024-3205(97)00669-3 [doi]
PST - ppublish
SO  - Life Sci. 1997;61(13):1245-51. doi: 10.1016/s0024-3205(97)00669-3.