PMID- 9325169
OWN - NLM
STAT- MEDLINE
DCOM- 19971027
LR  - 20171213
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 238
IP  - 3
DP  - 1997 Sep 29
TI  - cDNA cloning of a short type of multidrug resistance protein homologue, SMRP, 
      from a human lung cancer cell line.
PG  - 790-4
AB  - Members of the ATP binding cassette (ABC) superfamily are involved in the 
      energy-dependent transport of a wide variety of substrates including anticancer 
      agents across the membranes. We have cloned a cDNA fragment including a novel ABC 
      sequence from a cisplatin-resistant human lung adenocarcinoma cell line, 
      PC-14/CDDP, by reverse transcription polymerase chain reaction (RT-PCR) using 
      degenerate primers and screened a cDNA library using the cDNA fragment as a 
      probe. A full-length cDNA clone, BM4.8, was obtained. Sequence analysis showed 
      that the cDNA encoded a short type of multidrug resistance protein homologue, 
      SMRP, by computed homology search. SMRP was composed of 946 amino acids and had 
      two ABCs with walker A and B motifs. This gene was mapped on chromosome 3 at band 
      q27 by fluorescence in situ hybridization (FISH) analysis and was found to be 
      expressed in various tissues by Northern blot analysis.
FAU - Suzuki, T
AU  - Suzuki T
AD  - Pharmacology Division, National Cancer Center Research Institute, Tokyo, Japan.
FAU - Nishio, K
AU  - Nishio K
FAU - Sasaki, H
AU  - Sasaki H
FAU - Kurokawa, H
AU  - Kurokawa H
FAU - Saito-Ohara, F
AU  - Saito-Ohara F
FAU - Ikeuchi, T
AU  - Ikeuchi T
FAU - Tanabe, S
AU  - Tanabe S
FAU - Terada, M
AU  - Terada M
FAU - Saijo, N
AU  - Saijo N
LA  - eng
SI  - GENBANK/AB005659
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (DNA, Complementary)
RN  - 0 (Multidrug Resistance-Associated Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - Q20Q21Q62J (Cisplatin)
SB  - IM
MH  - ATP-Binding Cassette Transporters/*genetics/isolation & purification
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Carcinoma, Non-Small-Cell Lung/chemistry/*genetics
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 3
MH  - Cisplatin/pharmacology
MH  - Cloning, Molecular
MH  - DNA, Complementary/*isolation & purification
MH  - *Drug Resistance, Multiple
MH  - Humans
MH  - Lung Neoplasms/chemistry/*genetics
MH  - Molecular Sequence Data
MH  - Multidrug Resistance-Associated Proteins
MH  - Multigene Family
MH  - Neoplasm Proteins/*genetics/isolation & purification
MH  - Polymerase Chain Reaction
MH  - Tumor Cells, Cultured
EDAT- 1997/10/23 00:00
MHDA- 1997/10/23 00:01
CRDT- 1997/10/23 00:00
PHST- 1997/10/23 00:00 [pubmed]
PHST- 1997/10/23 00:01 [medline]
PHST- 1997/10/23 00:00 [entrez]
AID - S0006-291X(97)97346-0 [pii]
AID - 10.1006/bbrc.1997.7346 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1997 Sep 29;238(3):790-4. doi: 
      10.1006/bbrc.1997.7346.