PMID- 9325580
OWN - NLM
STAT- MEDLINE
DCOM- 19980202
LR  - 20190729
IS  - 1060-3271 (Print)
IS  - 1060-3271 (Linking)
VI  - 80
IP  - 5
DP  - 1997 Sep-Oct
TI  - Liquid chromatographic determination of safrole in sassafras-derived herbal 
      products.
PG  - 1023-8
AB  - A liquid chromatographic (LC) method was developed for determining safrole in 
      herbal products derived from sassafras (Sassafras albidum), as well as related 
      compounds such as isosafrole and dihydrosafrole. The procedure involves solvent 
      extraction and isolation of analyte by reversed-phase LC with UV detection at 235 
      nm. Safrole is resolved from related compounds and other sample constituents 
      including thymol, a component of thyme. A linear concentration range of 
      0.003-0.200 mg/mL was obtained for safrole, isosafrole, and dihydrosafrole. 
      Limits of detection (LOD) and quantitation (LOQ) were e0.0015 and 0.0051 
      micrograms/mL for safrole, 0.0018 and 0.0061 micrograms/mL for isosafrole, and 
      0.0038 and 0.0125 micrograms/mL for dihydrosafrole, respectively. Intraday 
      relative standard deviations (RSDs) for safrole (n = 5) from various samples 
      ranged from 1.30 to 5.39% at analyte levels of 0.01-1.5%. Safrole contents of 26 
      samples including root bark powder, leaves, oils, tea concentrate, herbal extract 
      tinctures, and herbal powder capsules ranged from < LOD for most leaf samples to 
      92.4% for an oil. Recoveries of safrole from fortified samples ranged from 83.6% 
      for an oil to 117.2% for a tincture preparation. Safrole contents of 0.09-4.66 
      mg/cup were found for brewed teas prepared from sassafras root bark powders and 
      tinctures.
FAU - Carlson, M
AU  - Carlson M
AD  - U.S. Food and Drug Administration, Minneapolis, MN 55401, USA.
FAU - Thompson, R D
AU  - Thompson RD
LA  - eng
PT  - Journal Article
PL  - England
TA  - J AOAC Int
JT  - Journal of AOAC International
JID - 9215446
RN  - 2ML63PLH7G (dihydrosafrole)
RN  - RSB34337V9 (Safrole)
RN  - W6337429LF (isosafrole)
SB  - IM
MH  - *Chromatography, Liquid
MH  - Isomerism
MH  - Lauraceae/*chemistry
MH  - *Phytotherapy
MH  - Reproducibility of Results
MH  - Safrole/analogs & derivatives/*analysis
MH  - Spectrophotometry, Ultraviolet
EDAT- 1997/11/05 00:00
MHDA- 1997/11/05 00:01
CRDT- 1997/11/05 00:00
PHST- 1997/11/05 00:00 [pubmed]
PHST- 1997/11/05 00:01 [medline]
PHST- 1997/11/05 00:00 [entrez]
PST - ppublish
SO  - J AOAC Int. 1997 Sep-Oct;80(5):1023-8.