PMID- 9326759
OWN - NLM
STAT- MEDLINE
DCOM- 19971212
LR  - 20191102
IS  - 0927-3948 (Print)
IS  - 0927-3948 (Linking)
VI  - 5
IP  - 3
DP  - 1997 Sep
TI  - Determinations of serum tumor necrosis factor alpha in corneal allografts.
PG  - 149-55
AB  - Corneal allograft rejection culminates in a series of interactions between 
      different classes of antigen presenting cells, cytokines and leukocytes. Tumor 
      necrosis factor-alpha (TNF-alpha) was recently reported to be elevated in acute 
      rejection of solid organ transplants. This cytokine is released early in immune 
      activation and may be detected in the peripheral circulation. Serial 
      determinations of TNF-alpha serum levels were performed following experimental 
      corneal allografts. Lewis rats received 3.5 mm orthotopic corneal grafts of 
      MHC-incompatible Wistar-Furth donors. TNF-alpha concentrations were measured in 
      serum samples collected pre- and postoperatively and measured by micro ELISA. 
      Clinical observations revealed graft rejection in 65.5% of corneal transplants 14 
      +/- 4 days following grafting. The mean serum level of TNF-alpha in control 
      animals without corneal graft (group I) was 41 +/- 12 pg/ml. Animals following 
      keratoplasty without allograft rejection (group II) showed a mean TNF-alpha level 
      of 54 +/- 16 pg/ml that did not differ from group I. The rejection group III 
      displayed significantly higher TNF-alpha levels (98 +/- 16 pg/ml, p < 0.05). 
      These significantly elevated levels were found even before the diagnosis of 
      rejection was established by clinical criteria. These data suggest systemic 
      immunoreactivity to corneal allografts. Elevated levels of cytokines may provide 
      valuable information in recipients undergoing rejection and may also provide a 
      rationale for systemic immunotherapy in some instances.
FAU - Pleyer, U
AU  - Pleyer U
AD  - Jules Stein Eye Institute, UCLA School of Medicine, USA.
FAU - Milani, J K
AU  - Milani JK
FAU - Ruckert, D
AU  - Ruckert D
FAU - Rieck, P
AU  - Rieck P
FAU - Mondino, B J
AU  - Mondino BJ
LA  - eng
GR  - EYO 4606/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Ocul Immunol Inflamm
JT  - Ocular immunology and inflammation
JID - 9312169
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Cornea/*chemistry
MH  - *Corneal Transplantation
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Graft Rejection/blood
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Rats, Inbred WF
MH  - Tumor Necrosis Factor-alpha/*analysis
EDAT- 1997/11/05 00:00
MHDA- 1997/11/05 00:01
CRDT- 1997/11/05 00:00
PHST- 1997/11/05 00:00 [pubmed]
PHST- 1997/11/05 00:01 [medline]
PHST- 1997/11/05 00:00 [entrez]
AID - 10.3109/09273949709116889 [doi]
PST - ppublish
SO  - Ocul Immunol Inflamm. 1997 Sep;5(3):149-55. doi: 10.3109/09273949709116889.