PMID- 9327736
OWN - NLM
STAT- MEDLINE
DCOM- 19971030
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 151
IP  - 4
DP  - 1997 Oct
TI  - Detection of numerical and structural alterations and fusion of chromosomes 16 
      and 1 in low-grade papillary breast carcinoma by fluorescence in situ 
      hybridization.
PG  - 1027-34
AB  - Intracystic papillary breast tumors, including intraductal papilloma and 
      low-grade intracystic papillary carcinoma, constitute a group for which 
      differential diagnosis is frequently difficult. We examined the status of 
      chromosomes 16 and 1 by multicolor fluorescence in situ hybridization (FISH) 
      analyses and the DNA ploidy patterns by flow cytometry in 26 intracystic 
      papillary tumors. Alterations of chromosomes 16 and 1 were detected by FISH in 93 
      and 85%, respectively, of 14 low-grade papillary carcinomas, and the latter 
      alterations always concurred with the former. Two-color FISH using probes for the 
      D1Z1 (1q12) and D16Z2 (16cen) loci or the D1Z1 and D16Z3 (16q11) loci showed that 
      fusion of chromosomes 16 and 1, mostly with breakpoints distal to 16q11.2 and 
      proximal to 1q12, occurred in 77% of the papillary carcinomas. DNA aneuploidy was 
      detected in 6% of these carcinomas. No papilloma showed these chromosome 
      alterations or DNA aneuploidy. Chromosome 16 and 1 fusions appeared to occur 
      frequently in diploid breast carcinomas and to be involved in the acquisition of 
      a malignant phenotype by duct epithelial cells. We suggest that two-color FISH 
      methods for detecting 1;16 fusions might be applicable as supportive methods for 
      the differential diagnosis of intracystic papillary breast tumors.
FAU - Tsuda, H
AU  - Tsuda H
AD  - Pathology Division, National Cancer Center Research Institute and Hospital, 
      Tokyo, Japan.
FAU - Takarabe, T
AU  - Takarabe T
FAU - Susumu, N
AU  - Susumu N
FAU - Inazawa, J
AU  - Inazawa J
FAU - Okada, S
AU  - Okada S
FAU - Hirohashi, S
AU  - Hirohashi S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - *Artificial Gene Fusion
MH  - Breast Neoplasms/*genetics/pathology
MH  - Carcinoma, Intraductal, Noninfiltrating/*genetics/pathology
MH  - Carcinoma, Papillary/*genetics/pathology
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 1/*genetics
MH  - Chromosomes, Human, Pair 16/*genetics
MH  - DNA Probes
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Loss of Heterozygosity
MH  - Ploidies
PMC - PMC1858061
EDAT- 1997/11/05 00:00
MHDA- 1997/11/05 00:01
PMCR- 1998/04/01
CRDT- 1997/11/05 00:00
PHST- 1997/11/05 00:00 [pubmed]
PHST- 1997/11/05 00:01 [medline]
PHST- 1997/11/05 00:00 [entrez]
PHST- 1998/04/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1997 Oct;151(4):1027-34.