PMID- 9328162
OWN - NLM
STAT- MEDLINE
DCOM- 19971113
LR  - 20190512
IS  - 0143-3334 (Print)
IS  - 0143-3334 (Linking)
VI  - 18
IP  - 9
DP  - 1997 Sep
TI  - Induction of chromosome-specific aneuploidy and micronuclei in human lymphocytes 
      by metabolites of 1,3-butadiene.
PG  - 1687-93
AB  - 1,3-Butadiene is a carcinogen in rodents, but its potential carcinogenicity to 
      humans remains controversial. Numerous studies have shown that butadiene and its 
      metabolites cause sister chromatid exchanges in vitro and in vivo. To test for 
      other types of genotoxicity, the micronucleus assay and fluorescence in situ 
      hybridization (FISH) have been used to detect chromosome damage in human 
      lymphocytes caused by two reactive metabolites of butadiene, diepoxybutane (DEB) 
      and monoepoxybutene (MEB). DEB (0.5-5.0 microM) significantly increased 
      micronucleus formation 4- to 6-fold (P <0.01) and MEB (1-500 microM) by 2- to 
      4-fold (P <0.01) over control levels. The ability of DEB and MEB to induce 
      aneuploidy of chromosomes 7, 8, 12, and X was examined using dual-color FISH in 
      both interphase and metaphase cells. These chromosomes were chosen because of 
      their involvement in leukemogenesis. Both DEB and MEB caused dose-dependent 
      increases in hyperdiploidy of chromosomes 12 and X, but had no discernible effect 
      on chromosomes 7 and 8. These results suggest that DEB and MEB cause 
      chromosome-specific aneuploidy in human cells. If formed in sufficient amounts, 
      DEB and MEB may produce chromosome damage of the type found in leukemia following 
      exposure to butadiene.
FAU - Xi, L
AU  - Xi L
AD  - Division of Environmental Health Sciences, School of Public Health, University of 
      California, Berkeley 94720-7360, USA.
FAU - Zhang, L
AU  - Zhang L
FAU - Wang, Y
AU  - Wang Y
FAU - Smith, M T
AU  - Smith MT
LA  - eng
GR  - P42 ES004705/ES/NIEHS NIH HHS/United States
GR  - B118-05/PHS HHS/United States
GR  - P42ES04705/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Carcinogenesis
JT  - Carcinogenesis
JID - 8008055
RN  - 0 (Butadienes)
RN  - 0 (Carcinogens)
RN  - 0 (Epoxy Compounds)
RN  - 478ERR5NKR (3,4-epoxy-1-butene)
RN  - 60OB65YNAB (diepoxybutane)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - JSD5FGP5VD (1,3-butadiene)
SB  - IM
MH  - *Aneuploidy
MH  - Butadienes/metabolism
MH  - Carcinogens/*toxicity
MH  - Cells, Cultured
MH  - Chromosomes, Human, Pair 12
MH  - Chromosomes, Human, Pair 7
MH  - Diploidy
MH  - Epoxy Compounds/*toxicity
MH  - Genotype
MH  - Glutathione Transferase/genetics
MH  - Humans
MH  - Interphase
MH  - Lymphocytes/*drug effects/ultrastructure
MH  - Male
MH  - Metaphase
MH  - *Micronuclei, Chromosome-Defective
MH  - Micronucleus Tests
EDAT- 1997/11/05 00:00
MHDA- 1997/11/05 00:01
CRDT- 1997/11/05 00:00
PHST- 1997/11/05 00:00 [pubmed]
PHST- 1997/11/05 00:01 [medline]
PHST- 1997/11/05 00:00 [entrez]
AID - 10.1093/carcin/18.9.1687 [doi]
PST - ppublish
SO  - Carcinogenesis. 1997 Sep;18(9):1687-93. doi: 10.1093/carcin/18.9.1687.