PMID- 9328573
OWN - NLM
STAT- MEDLINE
DCOM- 19971023
LR  - 20190723
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 206
IP  - 1-2
DP  - 1997 Aug 7
TI  - Characterization of anti-human interleukin-18 (IL-18)/interferon-gamma-inducing 
      factor (IGIF) monoclonal antibodies and their application in the measurement of 
      human IL-18 by ELISA.
PG  - 107-13
AB  - Interleukin-18 (IL-18)/interferon-gamma-inducing factor (IGIF) is a novel 
      cytokine, which is a potent inducer of IFN-gamma production and plays an 
      important role in Th1 responses. In order to develop a specific ELISA for the 
      measurement of human IL-18, we established 13 anti-human IL-18 monoclonal 
      antibodies and characterized them. 7 murine anti-human IL-18 mAbs and 6 rat 
      anti-human IL-18 mAbs were obtained by fusion of splenocytes from mice or rats 
      immunized with human IL-18, with SP2/0 myeloma cells. These antibodies were 
      classified into 4 groups according to competitive binding ELISAs to the human 
      IL-18 molecule. 1 murine mAb and all 6 rat mAbs neutralized IFN-gamma production 
      induced by IL-18. A specific human IL-18 ELISA was developed using two 
      neutralizing mAbs (#125-2H and #159-12B). This ELISA detects human IL-18 with a 
      minimum detection limit of 10 pg/ml, but does not react with heat-denatured human 
      IL-18. The ELISA does not show any cross-reactivity with other cytokines. Using 
      this assay, human IL-18 was measurable in the plasma of leukemia patients. This 
      ELISA would become a powerful tool for investigating the relationship between 
      IL-18 and various diseases or analyzing the control mechanisms of IL-18 
      production from IL-18 producing cells.
FAU - Taniguchi, M
AU  - Taniguchi M
AD  - Fujisaki Institute, Hayashibara Biochemical Laboratories Inc., Okayama, Japan. 
      fujih@po.harenet.or.jp
FAU - Nagaoka, K
AU  - Nagaoka K
FAU - Kunikata, T
AU  - Kunikata T
FAU - Kayano, T
AU  - Kayano T
FAU - Yamauchi, H
AU  - Yamauchi H
FAU - Nakamura, S
AU  - Nakamura S
FAU - Ikeda, M
AU  - Ikeda M
FAU - Orita, K
AU  - Orita K
FAU - Kurimoto, M
AU  - Kurimoto M
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-18)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Antibodies, Monoclonal/*chemistry
MH  - Cell Line
MH  - Cross Reactions
MH  - Cytokines/*blood/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Interferon-gamma/*biosynthesis
MH  - Interleukin-18
MH  - Leukemia/blood/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Rabbits
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1997/08/07 00:00
MHDA- 1997/10/27 00:01
CRDT- 1997/08/07 00:00
PHST- 1997/08/07 00:00 [pubmed]
PHST- 1997/10/27 00:01 [medline]
PHST- 1997/08/07 00:00 [entrez]
AID - S0022-1759(97)00094-X [pii]
AID - 10.1016/s0022-1759(97)00094-x [doi]
PST - ppublish
SO  - J Immunol Methods. 1997 Aug 7;206(1-2):107-13. doi: 
      10.1016/s0022-1759(97)00094-x.